Immunohistochemistry (Frozen sections) abreview for Anti-DDDDK tag antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - Triton x-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/500

Additional data

Additional Notes
Non-specific bimding is conspicuous in control ( not AAV9-FLAG treated mouse brain ) section. In the" ab1162" free section, non-specific binding is not seen after 2nd ab treatment.

久仁子 島崎

Verified customer

投稿 Sep 05 2016

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