Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-DDB1 抗体 [EPR6089] (ab109027)

Submit a review Q&A (1)References (18)
Western blot - Anti-DDB1 antibody [EPR6089] (ab109027)
  • Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (ab109027)
  • Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)
  • OI-RD Scanning - Anti-DDB1 antibody [EPR6089] (ab109027)
  • Anti-DDB1 antibody [EPR6089] (ab109027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6089] to DDB1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

製品の概要

  • 製品名

    Anti-DDB1 antibody [EPR6089]
    DDB1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6089] to DDB1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, HeLa, NIH3T3, and Human platelet lysates, Human breast tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: UV-treated HeLa

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109027の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
ICC/IF
1/100.
WB
1/50000 - 1/200000. Detects a band of approximately 130 kDa (predicted molecular weight: 127 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Belongs to the DDB1 family.

  • 翻訳後修飾

    Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.

  • 細胞内局在

    Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Damage specific DNA binding protein 1 antibody
    • Damage-specific DNA-binding protein 1 antibody
    • DDB 1 antibody
    • DDB p127 subunit antibody
    • Ddb1 antibody
    • DDB1_HUMAN antibody
    • DDBa antibody
    • DNA damage binding protein 1 antibody
    • DNA damage-binding protein 1 antibody
    • DNA damage-binding protein a antibody
    • HBV X-associated protein 1 antibody
    • UV damaged DNA binding factor antibody
    • UV damaged DNA binding protein 1 antibody
    • UV DDB 1 antibody
    • UV DDB1 antibody
    • UV-damaged DNA-binding factor antibody
    • UV-damaged DNA-binding protein 1 antibody
    • UV-DDB 1 antibody
    • X associated protein 1 antibody
    • XAP 1 antibody
    • XAP-1 antibody
    • XAP1 antibody
    • Xeroderma pigmentosum group E complementing protein antibody
    • Xeroderma pigmentosum group E-complementing protein antibody
    • XPCe antibody
    • XPE antibody
    • XPE BF antibody
    • XPE binding factor antibody
    • XPE-BF antibody
    • XPE-binding factor antibody
    see all

画像

  • Western blot - Anti-DDB1 antibody [EPR6089] (ab109027)
    Western blot - Anti-DDB1 antibody [EPR6089] (ab109027)
    All lanes : Anti-DDB1 antibody [EPR6089] (ab109027) at 1/50000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH3T3 cell lysate
    Lane 4 : Human platelet lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 127 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)
    Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)

    Immunocytochemistry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling DDB1 with ab109027 at 1/250 (8.9 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.

    Confocal image showing nuclear and cytoplasmic staining in NIH/3T3 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (ab109027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody [EPR6089] (ab109027)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109027 at a dilution of 1/100. Antigen retrieval was heat mediated before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)
    Immunocytochemistry/ Immunofluorescence - Anti-DDB1 antibody [EPR6089] (ab109027)

    ab109027 stained UV-treated HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109027 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • OI-RD Scanning - Anti-DDB1 antibody [EPR6089] (ab109027)
    OI-RD Scanning - Anti-DDB1 antibody [EPR6089] (ab109027)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-DDB1 antibody [EPR6089] (ab109027)
    Anti-DDB1 antibody [EPR6089] (ab109027)

参考文献 (18)

ab109027 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109027 は 18 報の論文で使用されています。

  • Luo Q  et al. OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Adv Sci (Weinh) 8:2002874 (2021). PubMed: 33898171
  • Wang X  et al. DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. Cell Rep 35:109281 (2021). PubMed: 34161765
  • Kim SA  et al. Aryl Sulfonamides Induce Degradation of Aryl Hydrocarbon Receptor Nuclear Translocator through CRL4DCAF15 E3 Ligase. Mol Cells 43:935-944 (2020). PubMed: 33168788
  • Reichermeier KM  et al. PIKES Analysis Reveals Response to Degraders and Key Regulatory Mechanisms of the CRL4 Network. Mol Cell 77:1092-1106.e9 (2020). PubMed: 31973889
  • Okur MN  et al. Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation. Nucleic Acids Res 48:2473-2485 (2020). PubMed: 31970402
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Bonjour,
La séquence que j'ai obtenue sur pubmed est la suivante:
MSYNYVVTAQKPTAVNACVTGHFTSEDDLNLLIAKNTRLEIYVVTPEGLRPVKEVGMYGKIAVMELFRPK
GESKDLLFILTAKYNACILEYKQSGDSIDIITRAHGNVQDRIGRPSETGIIGIIDPDCRMIGLRLYDGLF
KVIPLERDNKELKAFNIRLEELHVIDVKFLYSCQAPTICFVYQDPQGRHVKTYEVSLREKEFSKGPWKQE
NVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDVNGSRYLLGDMEGRL
FMLLLEKEEQMDGSVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLTTESNEQGSYVVVM
ETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRVAADRD
TDDTLVLSFVGQTRVLTLTGEEVEETDLAGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQNPQNLVSEW
KEPQGRKVSVCSCNSRQVLLAVGRVLYYLEIHPGELRQTSCTEMEHEVACLDVTPLGGNDTLSSLCAIGL
WTDISARILSLPGFQLLHKEMLGGEIIPRSILMTSFESSHYLLCALGDGALFYFSLNTDTGLLSDRKKVT
LGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSEGYPDSLALANNSTLT
IGTIDEIQKLHIRTVPLFESPRKICYQEVSQCFGVLSSRIEVQDASGGSSPLRPSASTQALSSSVSCSKL
FSGSTSPHETSFGEEVEVHNLLIIDQHTFEVLHTHQFLQNEYTLSLVSCKLGKDPTTYFVVGTAMVYPDE
AEPKQGRIVVFQYNDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTAEKELRTECNHYNNIM
ALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKD
SAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSPPTQGSVLFGTVNGMIGLVTSLSESWYNL
LLDVQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVIANLQIDDGSGM
KRETTVDDLIKVVEELTRIH

Je souhaite utiliser l'anticorps en WB et suivant le résultat obtenu, en IP.
Cordialement

Read More
Answer

Merci de votre intérêt pour ab21080 et merci pour m'avoir fourni la séquence de la DDB1 du xénope.

Nous avons à notre catalogue deux anticorps anti-DDB1 testés en Western Blot et en IP; le polyclonal de lapin ab21080 (https://www.abcam.com/ab21080) et le monoclonal de lapin ab109027 (https://www.abcam.com/ab109027).
Ces deux anticorps sont dirigés vers la partie C-terminale de la DDB1 humaine. Les 30 acides aminés de la DDB1 humaine présentent 90% d'homologie avec la séquence de la DDB1 du xénope. Ces 2 anticorps ont donc de bonnes chances de fonctionner chez le xénope.

Ces deux anticorps n’ont pas encore été testés chez le xénope et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires.
Cependant, si vous souhaitez tester ab21080 ou ab109027 chez le xénope, il me sera possible de vous offrir une remise.
Après soumission d’une Abreview avec vos résultats, un anticorps primaire de votre choix vous sera offert.

Etapes à suivre :

1. Nous confirmer que vous souhaiteriez tester ab21080 ou ab109027 chez le xénope afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab21080 ou ab109027

2. Acheter ab21080 ou ab109027 par téléphone, fax ou internet (https://www.abcam.com)

3. Le tester chez le xénope

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab21080 ou ab109027 ne pas fonctionne pas chez le xénope, après soumission de votre Abreview, vous recevrez quand même gratuitement un anticorps primaire de votre choix.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : https://www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com