Anti-DDB1 抗体 (ab9194)
Key features and details
- Goat polyclonal to DDB1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DDB1 antibody
DDB1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DDB1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
Sequence:C-DLIKVVEELTRIH
(Peptide available asab23152) -
特記事項
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9194の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the DDB1 family. -
翻訳後修飾
Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
細胞内局在
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 511951 Cow
- Entrez Gene: 13194 Mouse
- SwissProt: A1A4K3 Cow
- SwissProt: Q3U1J4 Mouse
- Unigene: 289915 Mouse
- Unigene: 466856 Mouse
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別名
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
画像
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Western blot - Anti-DDB1 antibody (ab9194)
Goat polyclonal to DDB-1 (1
Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.µ g/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab9194 は 11 報の論文で使用されています。
- Yang C et al. Small molecule NSC1892 targets the CUL4A/4B-DDB1 interactions and causes impairment of CRL4DCAF4 E3 ligases to inhibit colorectal cancer cell growth. Int J Biol Sci 16:1059-1070 (2020). PubMed: 32140073
- Tong X et al. DDB1 E3 ligase controls dietary fructose-induced ChREBPa stabilization and liver steatosis via CRY1. Metabolism 107:154222 (2020). PubMed: 32246987
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Pines A et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . PubMed: 29531219
- Pines A et al. PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1. J Cell Biol 199:235-49 (2012). WB . PubMed: 23045548