Anti-DDB1 抗体 (ab9194)
Key features and details
- Goat polyclonal to DDB1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
製品の概要
-
製品名
Anti-DDB1 antibody
DDB1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DDB1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Cow, Dog
-
免疫原
Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
Sequence:C-DLIKVVEELTRIH
(Peptide available asab23152) -
特記事項
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the DDB1 family. -
翻訳後修飾
Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
細胞内局在
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
-
参照データベース
- Entrez Gene: 511951 Cow
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Omim: 600045 Human
- SwissProt: A1A4K3 Cow
- SwissProt: Q16531 Human
see all -
別名
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
画像
-
Western blot - Anti-DDB1 antibody (ab9194)Goat polyclonal to DDB-1 (1
Goat polyclonal to DDB-1 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.µ g/ml) staining of NSO lysate (1E5 cells per lane). Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
参考文献 (9)
ab9194 は 9 報の論文で使用されています。
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Pines A et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . PubMed: 29531219
- Pines A et al. PARP1 promotes nucleotide excision repair through DDB2 stabilization and recruitment of ALC1. J Cell Biol 199:235-49 (2012). WB . PubMed: 23045548
- Anvar SY et al. Deregulation of the ubiquitin-proteasome system is the predominant molecular pathology in OPMD animal models and patients. Skelet Muscle 1:15 (2011). ICC/IF ; Mouse . PubMed: 21798095
- Leung-Pineda V et al. DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress. Cancer Res 69:2630-7 (2009). WB ; Human . PubMed: 19276361
- Alekseev S et al. Cellular concentrations of DDB2 regulate dynamic binding of DDB1 at UV-induced DNA damage. Mol Cell Biol 28:7402-13 (2008). WB, ICC/IF ; Mouse . PubMed: 18936169
- Luijsterburg MS et al. Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC. J Cell Sci 120:2706-16 (2007). WB, ICC/IF ; Human . PubMed: 17635991
- Westbrook TF et al. A genetic screen for candidate tumor suppressors identifies REST. Cell 121:837-48 (2005). PubMed: 15960972
- Ulane CM et al. STAT3 ubiquitylation and degradation by mumps virus suppress cytokine and oncogene signaling. J Virol 77:6385-93 (2003). WB . PubMed: 12743296
