Anti-DDAH2 抗体 [EPR15508(B)] (ab184166)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15508(B)] to DDAH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DDAH2 antibody [EPR15508(B)]
DDAH2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15508(B)] to DDAH2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15508(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184166の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
組織特異性
Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. -
配列類似性
Belongs to the DDAH family. - Information by UniProt
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参照データベース
- Entrez Gene: 23564 Human
- Entrez Gene: 51793 Mouse
- Entrez Gene: 294239 Rat
- Omim: 604744 Human
- SwissProt: O95865 Human
- SwissProt: Q99LD8 Mouse
- SwissProt: Q6MG60 Rat
- Unigene: 247362 Human
see all -
別名
- DDAH antibody
- DDAH II antibody
- DDAH-2 antibody
see all
画像
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Lanes 1-7 : Anti-DDAH2 antibody [EPR15508(B)] (ab184166) at 1/1000 dilution (Purified)
Lanes 8-9 : Anti-DDAH2 antibody [EPR15508(B)] (ab184166) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : SW620 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6 : Mouse brain lysate
Lane 7 : Rat brain lysate
Lane 8 : Mouse heart lysate
Lane 9 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab184166 は 6 報の論文で使用されています。
- Li K et al. Cysteine-Rich Whey Protein Isolate (CR-WPI) Ameliorates Erectile Dysfunction by Diminishing Oxidative Stress via DDAH/ADMA/NOS Pathway. Oxid Med Cell Longev 2022:8151917 (2022). PubMed: 35355865
- Dague A et al. Maternal use of methamphetamine induces sex-dependent changes in myocardial gene expression in adult offspring. Physiol Rep 10:e15509 (2022). PubMed: 36426716
- Hannemann J et al. Upregulation of DDAH2 Limits Pulmonary Hypertension and Right Ventricular Hypertrophy During Chronic Hypoxia in Ddah1 Knockout Mice. Front Physiol 11:597559 (2020). PubMed: 33281630
- Badran M et al. Alpha Lipoic Acid Improves Endothelial Function and Oxidative Stress in Mice Exposed to Chronic Intermittent Hypoxia. Oxid Med Cell Longev 2019:4093018 (2019). PubMed: 31093313
- Yang RH et al. Interleukin enhancement binding factor 3 inhibits cardiac hypertrophy by targeting asymmetric dimethylarginine-nitric oxide. Nitric Oxide 93:44-52 (2019). PubMed: 31536826
- Wang H et al. DDAH1 plays dual roles in PM2.5 induced cell death in A549 cells. Biochim Biophys Acta 1860:2793-801 (2016). PubMed: 26996393