Anti-DDAH1 抗体 (ab2231)
製品の概要
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製品名
Anti-DDAH1 antibody
DDAH1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DDAH1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, ICC/IF, IHC-Fr, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Cow
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免疫原
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24663083 | |
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
組織特異性
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. -
配列類似性
Belongs to the DDAH family. - Information by UniProt
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参照データベース
- Entrez Gene: 23576 Human
- Entrez Gene: 64157 Rat
- Omim: 604743 Human
- SwissProt: P56965 Cow
- SwissProt: O94760 Human
- SwissProt: O08557 Rat
- Unigene: 379858 Human
- Unigene: 713411 Human
see all -
別名
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
see all
画像
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Western blot - Anti-DDAH1 antibody (ab2231)Anti-DDAH1 antibody (ab2231) at 0.100000001490116 µg/ml + Human kidney lysate at 35 µg
Predicted band size: 31 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DDAH1 antibody (ab2231)ICC/IF image of ab2231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH1 antibody (ab2231)IHC image of ab2231 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2231, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
参考文献
ab2231 は 13 報の論文で使用されています。
- Zhu ZD et al. DDAH2 alleviates myocardial fibrosis in diabetic cardiomyopathy through activation of the DDAH/ADMA/NOS/NO pathway in rats. Int J Mol Med 43:749-760 (2019). PubMed: 30569164
- Balasubramanian V et al. Post-Transcriptional Regulation of Hepatic DDAH1 with TNF Blockade Leads to Improved eNOS Function and Reduced Portal Pressure In Cirrhotic Rats. Sci Rep 7:17900 (2017). WB ; Rat . PubMed: 29263339
- Hulin JA et al. MiR-193b regulates breast cancer cell migration and vasculogenic mimicry by targeting dimethylarginine dimethylaminohydrolase 1. Sci Rep 7:13996 (2017). PubMed: 29070803
- Mookerjee RP et al. Hepatic dimethylarginine-dimethylaminohydrolase1 is reduced in cirrhosis and is a target for therapy in portal hypertension. J Hepatol 62:325-31 (2015). WB, IHC ; Human . PubMed: 25152204
- D'Mello R et al. Dimethylarginine dimethylaminohydrolase 1 is involved in spinal nociceptive plasticity. Pain 156:2052-60 (2015). WB, IHC-FoFr ; Rat . PubMed: 26098438
