Anti-DCUN1D1 抗体 (ab99506)
Key features and details
- Goat polyclonal to DCUN1D1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DCUN1D1 antibody
DCUN1D1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DCUN1D1 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow
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免疫原
Synthetic peptide:
C-RPQIAGTKSTT
, corresponding to C terminal amino acids 248-258 of Human DCUN1D1 (NP_065691.2). -
ポジティブ･コントロール
- HepG2 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 30 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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関連性
DCUN1D1 (DCUN1 domain-containing protein 1) contains a DUF298 domain and a UBA-like ubiquitin. Forms part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression. Defects in DCUN1D1 may be a cause of squamous cell carcinomas. Strongly overexpressed in thyroid tumors, bronchioloalveolar carcinomas, and malignant tissues of squamous cell carcinoma of the oral tongue. Not overexpressed in aggressive adrenocortical carcinomas. -
参照データベース
- Entrez Gene: 539869 Cow
- Entrez Gene: 54165 Human
- Entrez Gene: 114893 Mouse
- Omim: 605905 Human
- SwissProt: Q96GG9 Human
- SwissProt: Q9QZ73 Mouse
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別名
- DCN1 defective in cullin neddylation 1 domain containing 1 antibody
- DCN1 like protein 1 antibody
- DCNL1 antibody
see all
画像
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Western blot - Anti-DCUN1D1 antibody (ab99506)Anti-DCUN1D1 antibody (ab99506) at 0.5 µg/ml + HepG2 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 1 hour
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99506 は論文での使用が確認できていません。
