Anti-DCTN1/p150-glued 抗体 (ab11806)
Key features and details
- Goat polyclonal to DCTN1/p150-glued
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DCTN1/p150-glued antibody
DCTN1/p150-glued 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DCTN1/p150-glued -
由来種
Goat -
特異性
This antibody is expected to recognise both human isoforms. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Drosophila melanogaster
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免疫原
Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1266-1278 (C terminal).
Sequence:C-QEQLHQLHSRLIS
(Peptide available asab23214) -
ポジティブ･コントロール
- WB: Human cerebellum lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11806の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa.
A 1 hour primary incubation is recommended for this product. Approx 150kDa band observed in A549 and Human Testis lysates |
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 150 kDa. A 1 hour primary incubation is recommended for this product. Approx 150kDa band observed in A549 and Human Testis lysates |
ターゲット情報
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機能
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. -
組織特異性
Brain. -
関連疾患
Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. -
配列類似性
Belongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain. -
翻訳後修飾
Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 39536 Drosophila melanogaster
- Entrez Gene: 1639 Human
- Entrez Gene: 29167 Rat
- Omim: 601143 Human
- SwissProt: P13496 Drosophila melanogaster
- SwissProt: Q14203 Human
- SwissProt: P28023 Rat
- Unigene: 12980 Drosophila melanogaster
see all -
別名
- 150 kDa dynein associated polypeptide antibody
- 150 kDa dynein-associated polypeptide antibody
- DAP 150 antibody
see all
画像
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Western blot - Anti-DCTN1/p150-glued antibody (ab11806)Anti-DCTN1/p150-glued antibody (ab11806) at 1 µg/ml + human cerebellum lysate (RIPA buffer) at 35 µg
Predicted band size: 150 kDaDetected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (13)
ab11806 は 13 報の論文で使用されています。
- Castellano-Pellicena I et al. Melanin Distribution in Human Skin: Influence of Cytoskeletal, Polarity, and Centrosome-Related Machinery of Stratum basale Keratinocytes. Int J Mol Sci 22:N/A (2021). PubMed: 33808676
- Deshimaru M et al. DCTN1 Binds to TDP-43 and Regulates TDP-43 Aggregation. Int J Mol Sci 22:N/A (2021). PubMed: 33924373
- Tian WT et al. New phenotype of DCTN1-related spectrum: early-onset dHMN plus congenital foot deformity. Ann Clin Transl Neurol 7:200-209 (2020). PubMed: 32023010
- Lee M et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol 219:N/A (2020). PubMed: 31910261
- Lough KJ et al. Telophase correction refines division orientation in stratified epithelia. Elife 8:N/A (2019). PubMed: 31833472
