Key features and details
- Rabbit polyclonal to DcR1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
DcR1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to DcR1
アプリケーション適用あり: ICC/IF, WBmore details
種交差性交差種: Mouse, Rat, Human
- HeLa whole cell lysate.
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
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一次抗体 備考Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
Our Abpromise guarantee covers the use of ab2087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa. Can be blocked with DcR1 peptide (human).|
関連性DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
細胞内局在Cell Membrane and Cytoplasmic
- Antagonist decoy receptor for TRAIL/Apo 2L antibody
- CD263 antibody
- Cytotoxic TRAIL receptor 3 antibody
All lanes : Anti-DcR1 antibody (ab2087) at 1 µg/ml
Lane 1 : Hela cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat liver tissue lysate
Predicted band size: 65 kDa
Western blot analysis of DcR1 in HeLa cell (A), mouse (B) and rat (C) liver tissue lysates with anti-DcR1 (ED2) at 1
ab2087 at 10µg/ml staining DcR1 in rat liver tissue by ICC/IF
Immunofluorescence of DcR1 in Rat Liver cells using ab2087 at 20 ug/ml.
ab2087 は 5 報の論文で使用されています。
- Wang Z et al. Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. Signal Transduct Target Ther 5:40 (2020). PubMed: 32327638
- Rath C et al. Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers. Anal Biochem 583:113323 (2019). PubMed: 31129134
- Li N et al. The role of Zeb1 in the pathogenesis of morbidly adherent placenta. Mol Med Rep 20:2812-2822 (2019). PubMed: 31322233
- You C et al. ß-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol 53:2258-2268 (2018). PubMed: 30132512
- Ziauddin MF et al. TRAIL gene-armed oncolytic poxvirus and oxaliplatin can work synergistically against colorectal cancer. Gene Ther 17:550-9 (2010). PubMed: 20182517