Anti-DcR1 抗体 (ab2087)
Key features and details
- Rabbit polyclonal to DcR1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DcR1 antibody
DcR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DcR1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
CKEGTFRNENSPE
, corresponding to amino acids 111-123 of Human DcR1. -
ポジティブ･コントロール
- HeLa whole cell lysate.
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特記事項
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2087の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa. Can be blocked with DcR1 peptide (human).
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa. Can be blocked with DcR1 peptide (human). |
ターゲット情報
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関連性
DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis. -
細胞内局在
Cell Membrane and Cytoplasmic -
参照データベース
- Entrez Gene: 8794 Human
- Omim: 603613 Human
- SwissProt: O14798 Human
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別名
- Antagonist decoy receptor for TRAIL/Apo 2L antibody
- CD263 antibody
- Cytotoxic TRAIL receptor 3 antibody
see all
画像
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Western blot - Anti-DcR1 antibody (ab2087)All lanes : Anti-DcR1 antibody (ab2087) at 1 µg/ml
Lane 1 : Hela cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat liver tissue lysate
Predicted band size: 65 kDaWestern blot analysis of DcR1 in HeLa cell (A), mouse (B) and rat (C) liver tissue lysates with anti-DcR1 (ED2) at 1
µ g/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody (ab2087)ab2087 at 10µg/ml staining DcR1 in rat liver tissue by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody (ab2087)Immunofluorescence of DcR1 in Rat Liver cells using ab2087 at 20 ug/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab2087 は 6 報の論文で使用されています。
- Wang Z et al. Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia. Signal Transduct Target Ther 5:40 (2020). PubMed: 32327638
- Jin W et al. Ischemic Preconditioning Upregulates Decoy Receptors to Protect SH-SY5Y Cells from OGD Induced Cellular Damage by Inhibiting TRAIL Pathway and Agitating PI3K/Akt Pathway. Mol Neurobiol 57:3658-3670 (2020). PubMed: 32564286
- Rath C et al. Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers. Anal Biochem 583:113323 (2019). PubMed: 31129134
- Li N et al. The role of Zeb1 in the pathogenesis of morbidly adherent placenta. Mol Med Rep 20:2812-2822 (2019). PubMed: 31322233
- You C et al. ß-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol 53:2258-2268 (2018). PubMed: 30132512
- Ziauddin MF et al. TRAIL gene-armed oncolytic poxvirus and oxaliplatin can work synergistically against colorectal cancer. Gene Ther 17:550-9 (2010). PubMed: 20182517
