製品の概要
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製品名Anti-DcR1 antibody
DcR1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DcR1
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide:
CKEGTFRNENSPE
, corresponding to amino acids 111-123 of Human DcR1. -
ポジティブ･コントロール
- HeLa whole cell lysate.
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特記事項
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium azide
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Concentration information loading... -
精製度Affinity purified
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一次抗体 備考Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumour cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy-receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab2087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa.Can be blocked with DcR1 peptide (ab6096). Can be blocked with DcR1 peptide (human). |
ターゲット情報
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関連性DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
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細胞内局在Cell Membrane and Cytoplasmic
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参照データベース
- Entrez Gene: 8794 Human
- Omim: 603613 Human
- SwissProt: O14798 Human
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別名
- Antagonist decoy receptor for TRAIL/Apo 2L antibody
- CD263 antibody
- Cytotoxic TRAIL receptor 3 antibody
see all
画像
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Western blot - Anti-DcR1 antibody (ab2087)All lanes : Anti-DcR1 antibody (ab2087) at 1 µg/ml
Lane 1 : Hela cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat liver tissue lysate
Predicted band size: 65 kDaWestern blot analysis of DcR1 in HeLa cell (A), mouse (B) and rat (C) liver tissue lysates with anti-DcR1 (ED2) at 1
µ g/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody (ab2087)ab2087 at 10µg/ml staining DcR1 in rat liver tissue by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody (ab2087)Immunofluorescence of DcR1 in Rat Liver cells using ab2087 at 20 ug/ml.
参考文献
This product has been referenced in:
- Ziauddin MF et al. TRAIL gene-armed oncolytic poxvirus and oxaliplatin can work synergistically against colorectal cancer. Gene Ther 17:550-9 (2010). Read more (PubMed: 20182517) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"