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Anti-DCK 抗体 (ab96599)

Reviews (2)Q&A (1)References (6)
Western blot - Anti-DCK antibody (ab96599)
  • Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody (ab96599)

Key features and details

  • Rabbit polyclonal to DCK
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-DCK antibody
    DCK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to DCK

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow

  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 1 - 216 of Human DCK.

  • ポジティブ･コントロール

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lines.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab96599の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
ICC/IF
1/100 - 1/200.
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
ICC/IF
1/100 - 1/200.

ターゲット情報

  • 機能

    Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

  • 配列類似性

    Belongs to the DCK/DGK family.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • dCK antibody
    • DCK protein antibody
    • DCK_HUMAN antibody
    • Deoxycytidine kinase antibody
    • Deoxynucleoside kinase antibody
    • EC 2.7.1 antibody
    • EC 2.7.1.74 antibody
    • MGC117410 antibody
    • MGC138632 antibody
    • OTTHUMP00000219118 antibody
    • OTTHUMP00000219119 antibody
    see all

画像

  • Western blot - Anti-DCK antibody (ab96599)
    Western blot - Anti-DCK antibody (ab96599)
    Anti-DCK antibody (ab96599) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    12% SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody (ab96599)
    Immunocytochemistry/ Immunofluorescence - Anti-DCK antibody (ab96599)
    ab96599, at 1/200 dilution, staining DCK in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

参考文献 (6)

ab96599 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab96599 は 6 報の論文で使用されています。

  • Vena F  et al. Targeting Casein Kinase 1 Delta Sensitizes Pancreatic and Bladder Cancer Cells to Gemcitabine Treatment by Upregulating Deoxycytidine Kinase. Mol Cancer Ther 19:1623-1635 (2020). PubMed: 32430484
  • Hu Q  et al. dCK negatively regulates the NRF2/ARE axis and ROS production in pancreatic cancer. Cell Prolif 51:e12456 (2018). PubMed: 29701272
  • Zhong R  et al. Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells. Int J Oncol 52:1000-1010 (2018). PubMed: 29393406
  • Hammam K  et al. Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology. Nat Commun 8:1420 (2017). PubMed: 29127277
  • Kerr M  et al. Deoxycytidine kinase expression underpins response to gemcitabine in bladder cancer. Clin Cancer Res 20:5435-45 (2014). WB . PubMed: 25224279
  • Frese KK  et al. nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer. Cancer Discov 2:260-269 (2012). PubMed: 22585996

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DCK antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine pancreatic adenocarcinoma)
Specification
murine pancreatic adenocarcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kris Frese

Verified customer

投稿 Jan 23 2012

Western blot abreview for Anti-DCK antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (multiple tissues)
Loading amount
40 µg
Specification
multiple tissues
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kris Frese

Verified customer

投稿 Jan 06 2012

Question

o echnique does not require any optimization and it works very well with all the other antibodies we have, (including abcam) we tried some of your suggestions but without any successful result.
Increasing dilution of the secondary are not decreasing the unspecifity but only increasing the time of exposure to get visible bands.
Our secondary antibody does not create any unspecific binding with any other antibody it is Affinity purified goat anti-rabbit IgG (H&L) antibody conjugated to horseradish peroxidase.
At this point we have already wasted too much time and a lot of other reagents to run blots with different conditions which have shown unsuccessful results to the antibody.
We would like to receive these two substitutions as soon as possible.
Cda ab82346
Dck ab96599

Read More
Answer

rdetails and for your cooperation.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful so far. However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.
The details provided in the questionnaire enable us to closely monitor the quality of our products.
As requested, I have issued a free of charge replacement
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

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