Anti-DCAMKL1 抗体 [EPR6085] - BSA and Azide free (ab187184)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6085] to DCAMKL1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DCAMKL1 antibody [EPR6085] - BSA and Azide free
DCAMKL1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6085] to DCAMKL1 - BSA and Azide free -
由来種
Rabbit -
特異性
The human recommendation is based on the WB results. We do not guarantee IHC-P for human.
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アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate, Mouse and rat cerebllum lysate, brain lysate and colon lysate, NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, HAP1 whole cell lysate, human brain lysate. IHC-P: Mouse stomach, Rat colon.
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特記事項
ab187184 is the carrier-free version of ab109029.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6085 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The human recommendation is based on the WB results. We do not guarantee IHC-P for human. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The human recommendation is based on the WB results. We do not guarantee IHC-P for human. |
WB
Use at an assay dependent concentration. Detects a band of approximately 50-82 kDa (predicted molecular weight: 82 kDa). |
ターゲット情報
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機能
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. -
組織特異性
In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 2 doublecortin domains.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 9201 Human
- Entrez Gene: 13175 Mouse
- Entrez Gene: 83825 Rat
- Omim: 604742 Human
- SwissProt: O15075 Human
- SwissProt: Q9JLM8 Mouse
- SwissProt: O08875 Rat
- Unigene: 507755 Human
see all -
別名
- Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
- CL1 antibody
- CLICK1 antibody
see all
画像
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Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/5000 dilution + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 47,82 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The antibody recognizes isoforms of AL, AS, BS and BL.
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All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse cerebllum lysate
Lane 4 : Rat cerebllum lysate
Lane 5 : Mouse colon lysate
Lane 6 : Rat colon lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 47,82 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
This antibody recognizes isoforms of AL, AS, BS and BL.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029).
Immunohistochemical analysis of Paraffin-embedded sections rat colon tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat colon tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109029).
Immunohistochemical analysis of Paraffin-embedded sections mouse stomach tissue labelling DCAMKL1 with ab109029 at 1/4000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse stomach tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0)
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab187184 は論文での使用が確認できていません。