Anti-DC-SIGN 抗体 (ab5716)

Submit a review Q&A (1)References (2)

Key features and details

  • Rabbit polyclonal to DC-SIGN
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-DC-SIGN antibody
    DC-SIGN 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to DC-SIGN

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human DC-SIGN aa 277-293.
    (Peptide available as ab6092)

  • ポジティブ･コントロール

    • Human placenta tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.

  • 組織特異性

    Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.

  • 配列類似性

    Contains 1 C-type lectin domain.

  • ドメイン

    The tandem repeat domain, also called neck domain, mediates oligomerization.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C type lectin domain family 4 member L antibody
      • C-type lectin domain family 4 member L antibody
      • CD 209 antibody
      • CD209 antibody
      • CD209 antigen antibody
      • CD209 antigen-like protein A antibody
      • CD209 molecule antibody
      • CD209_HUMAN antibody
      • Cd209a antibody
      • CDSIGN antibody
      • CIRE antibody
      • CLEC4L antibody
      • DC SIGN antibody
      • DC SIGN1 antibody
      • DC-SIGN antibody
      • DC-SIGN1 antibody
      • DCSIGN antibody
      • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
      • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
      • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
      • Dengue fever, protection against, included antibody
      • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
      • HIV GP120 Binding Protein antibody
      • MGC129965 antibody
      • MGC130443 antibody
      • SIGN-R1 antibody
      • SIGNR5 antibody
      see all

    画像

    • Western blot - Anti-DC-SIGN antibody (ab5716)
      Western blot - Anti-DC-SIGN antibody (ab5716)

      ab5716 at 2 µg /ml staining approximately 44kDa of human CD209 in Human placenta tissue lysate by Western blot (ECL). Lane 1 absence of blocking peptide and Lane 2 presence of blocking peptide.

      ab5716 at 2 µg /ml staining approximately 44kDa of human CD209 in Human placenta tissue lysate by Western blot (ECL). Lane 1 absence of blocking peptide and Lane 2 presence of blocking peptide.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5716)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5716)
      Immunohistochemistry of DC-SIGN in human small intestine tissue with DC-SIGN antibody at 10 µg/ml.

    参考文献 (2)

    ab5716 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5716 は 2 報の論文で使用されています。

    • Zhang L  et al. Nab-paclitaxel is an active drug in preclinical model of pediatric solid tumors. Clin Cancer Res 19:5972-83 (2013). Human . PubMed: 23989978
    • Mikulak J  et al. DC-specific ICAM-3-grabbing nonintegrin mediates internalization of HIV-1 into human podocytes. Am J Physiol Renal Physiol 299:F664-73 (2010). WB ; Human . PubMed: 20630938

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Hello, I would like to know if your DC-SIGN antibody will block DC-SIGN's binding pocket, in otherword, can the antibody bind DC-SIGN while it is simultaneously binding a sugar? Thank you

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    Answer

    Thank you for your enquiry. This antibody was raised against residues 277 to 293 of human DC-SIGN. This region is part of the C-type Lectin Domain. Unfortunately, since this antibody is generated against a linear peptide from the protein, we do not know how the antibodies will bind to the protein in the native conformation and affect the binding sites. We apologise for the inconvenience,

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