Anti-DC-SIGN 抗体 (ab5715)
Key features and details
- Rabbit polyclonal to DC-SIGN
- Suitable for: ICC/IF, IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DC-SIGN antibody
DC-SIGN 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DC-SIGN -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human DC-SIGN aa 384-404.
(Peptide available asab6091) -
ポジティブ･コントロール
- Human placenta tissue lysate.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa. |
ターゲット情報
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機能
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. -
組織特異性
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. -
配列類似性
Contains 1 C-type lectin domain. -
ドメイン
The tandem repeat domain, also called neck domain, mediates oligomerization. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 30835 Human
- Omim: 604672 Human
- SwissProt: Q9NNX6 Human
- Unigene: 278694 Human
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別名
- C type lectin domain family 4 member L antibody
- C-type lectin domain family 4 member L antibody
- CD 209 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody (ab5715)Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 µg/ml. -
Western blot - Anti-DC-SIGN antibody (ab5715)ab5715 at 0.5
µ g /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL).
ab5715 at 0.5 µg /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL). -
Western blot - Anti-DC-SIGN antibody (ab5715)Lane 1 : Anti-DC-SIGN antibody (ab5715) at 1 µg/ml
Lane 2 : Anti-DC-SIGN antibody (ab5715) at 2 µg/ml
All lanes : Human placenta
Observed band size: 44 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody (ab5715)Immunofluorescence of DC-SIGN in Human Lymph Node cells using ab5715 at 20 ug/ml.
プロトコール
参考文献 (1)
ab5715 は 1 報の論文で使用されています。
- Moutsopoulos NM et al. Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss. Sci Transl Med 6:229ra40 (2014). PubMed: 24670684
