Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5635] to DBF4 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
製品名Anti-DBF4 antibody [EPR5635] - BSA and Azide free
DBF4 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5635] to DBF4 - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP
Synthetic peptide within Human DBF4 aa 1-100. The exact sequence is proprietary.
Database link: Q9UBU7
ab247972 is the carrier-free version of ab124707 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 2.49 x 10 -10 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab247972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.|
機能Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
組織特異性Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
配列類似性Contains 2 BRCT domains.
Contains 1 DBF4-type zinc finger.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Activator of S phase kinase antibody
- ASK antibody
- CHIF antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.