Anti-DBC-1 抗体 (ab70242)
Key features and details
- Rabbit polyclonal to DBC-1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-DBC-1 antibody
DBC-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DBC-1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human DBC-1 aa 875-923 (C terminal).
Database link: Q8N163 -
ポジティブ・コントロール
- Nuclear extract from HeLa cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70242の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
|
|
IP |
Use at 1-10 µg/mg of lysate.
|
特記事項 |
---|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). |
IP
Use at 1-10 µg/mg of lysate. |
ターゲット情報
-
機能
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. -
組織特異性
Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 464047 Chimpanzee
- Entrez Gene: 507114 Cow
- Entrez Gene: 486122 Dog
- Entrez Gene: 101135799 Gorilla
- Entrez Gene: 57805 Human
- Entrez Gene: 100173017 Orangutan
- Entrez Gene: 100155718 Pig
- Entrez Gene: 716288 Rhesus monkey
see all -
別名
- CCAR2 antibody
- Cell cycle and apoptosis regulator 2 antibody
- Cell division cycle and apoptosis regulator protein 2 antibody
see all
画像
-
All lanes : Anti-DBC-1 antibody (ab70242) at 1 µg/ml
Lane 1 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : Renca (mouse renal cell carcinoma cell line) whole cell lysate
Lane 3 : 4T1 (mouse mammary gland tumor cell line) whole cell lysate
Lane 4 : CT26 (mouse colon carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 103 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DBC-1 with ab70242 at 1/1000 (1µg/ml). Detection: DAB.
-
Lanes 1-2 : Anti-DBC-1 antibody (ab70242) at 1/1000 dilution
Lanes 3-4 : Anti-DBC-1 antibody (ab70242) at 1/10000 dilution
Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds -
Immunoprecipitation: ab70242 used at 1 µg/100 µg of nuclear extract from HeLa cells. Western blot: 1/1000 ab70242. Detection: Chemiluminescence with exposure times of 5 mins
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab70242 は 1 報の論文で使用されています。
- Raynes R et al. The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response. PLoS One 8:e54364 (2013). WB ; Human . PubMed: 23349863