Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (chicken 10-day embryo gonad, cryosection)
Permeabilization
Yes - 0.5% Triton-X100
Specification
chicken 10-day embryo gonad, cryosection
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Cy3 Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Picture legend:
DAZL (red)
DNA labeling with TO-PRO-3 (cyan)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 25 2021