Anti-DAPK2 抗体 (ab8404)
Key features and details
- Rabbit polyclonal to DAPK2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DAPK2 antibody
DAPK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DAPK2 -
由来種
Rabbit -
特異性
Does not react with DAPK1. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human DAPK2 aa 356-370.
Sequence:ARRKALHPRRRSSTS
(Peptide available asab8436) -
ポジティブ・コントロール
- A431 whole cell lysate. An approximately42 kDa band can be detected.
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特記事項
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types ofcells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family ofserine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1).
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Affinity purified -
一次抗体 備考
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types ofcells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family ofserine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8404の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 2 µg/ml.
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|
WB |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
Use a concentration of 2 µg/ml. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. -
組織特異性
Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 23604 Human
- Entrez Gene: 13143 Mouse
- Entrez Gene: 300799 Rat
- Omim: 604727 Human
- SwissProt: Q9UIK4 Human
- SwissProt: Q8VDF3 Mouse
- Unigene: 237886 Human
- Unigene: 304472 Mouse
see all -
別名
- DAK2 antibody
- DAP kinase 2 antibody
- DAP kinase related protein 1 antibody
see all
画像
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All lanes : Anti-DAPK2 antibody (ab8404) at 1 µg/ml
Lane 1 : A431 whole cell lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat kidney lysate
Observed band size: 42 kDa why is the actual band size different from the predicted? -
ab8404 at 2µg/ml staining DAPK2 in mouse spleen cells by IHC
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab8404 は論文での使用が確認できていません。