アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    dab-substrate-kit-ab64238.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Tools and Reagents IHC Tools/ Reagents
Share by email

DAB Substrate Kit (ab64238)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (6)Q&A (18)References (72)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

こちらの製品もご検討ください

ブロッキング
Product image
Hydrogen Peroxide Blocking Reagent (ab64218)

関連製品

製品の概要

  • 製品名

    DAB Substrate Kit
  • 製品の概要

    DAB substrate kit ab64238 is used for IHC staining using HRP / peroxidase enzyme for detection. Reagent is stable for up to 6 hours after mixing the two components.


    DAB (3,3'Diaminobenzidine) is the most commonly used chromogen for immunohistochemical staining. In the presence of HRP / peroxidase, DAB produces a brown precipitate that is insoluble in alcohol.


    This product is a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate.  


    IHC protocol suitable for use with DAB substrate kit:
    For frozen sections, skip steps 1 and 2. 


    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.


    2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.


    3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.


    4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.


    5. Apply primary antibody in antibody diluent and incubate.


    6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.


    7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.


    8. Rinse 4 times in buffer. Place slide in DAB substrate ab64238 or AEC substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.


    9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.


    10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.

  • 特記事項

    Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • アプリケーション

    適用あり: IHC-Pmore details

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • バッファー

    Constituents: 100% Propylene glycol, 5% Hydrogen peroxide, DAB substrate buffer
  • 内容 60 ml 125 ml
    50x DAB Chromogen 1 x 2ml 1 x 4ml
    DAB substrate 1 x 60ml 1 x 125ml
  • 研究分野

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • DAB Substrate
  • 関連性

    3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.
  • 別名

    • 3 3' Diaminobenzidine

関連製品

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64238 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P
    Use at an assay dependent dilution.
    特記事項
    IHC-P
    Use at an assay dependent dilution.

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.

      Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.

      Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Kumar P et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immuno-staining of formalin–fixed and paraffin-embedded liver sections with ab64238. Secontions were obtained from control (saline) and treated with CCl4+CT-N and CCl4+AD-N (left) and antibody control (right). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)Jujo T et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of mouse lung. Samples were fixed in 10% buffered formalin, paraffinized and sliced at 1.5 µm thick. Antigen retrieval was performed using ab64214 for the deparaffinized slices. Sections were blocked with 2% normal goat serum, PBS(-) and 0.1% Tween20. They were then incubated with the primary antibodies for 1 hour at 4°C and with secondary antibodies for 30 minutes at room temperature. The avidin-biotin-peroxidase complex method with peroxidase streptavidin and the DAB substrate kit ab64238 was performed.

      (A) A resected lung from a mouse sacrificed 28 days after SCL injection.

      (B) Haemotoxylin and Eosin staining showing the tumor composed of a central area with necrosis and a peripheral zone filled with SCLs.

      (C) Immunohistochemical staining for MMP-14 showing positive expression of MMP-14 in the peripheral zone of the tumor and negative in central zone.

      (D) Immunohistochemical staining weak staining of MMP-2 in the peripheral zone. 

    プロトコール

    • DAB Substrate Kit
    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (72)

    ab64238 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab64238 は 72 報の論文で使用されています。

    • Gualandi M  et al. Oncogenic ALKF1174L drives tumorigenesis in cutaneous squamous cell carcinoma. Life Sci Alliance 3:N/A (2020). PubMed: 32312912
    • Albarracin L  et al. Efficient Selection of New Immunobiotic Strains With Antiviral Effects in Local and Distal Mucosal Sites by Using Porcine Intestinal Epitheliocytes. Front Immunol 11:543 (2020). PubMed: 32322251
    • Jiang W  et al. Long non-coding RNA EBLN3P promotes the recovery of the function of impaired spiral ganglion neurons by competitively binding to miR-204-5p and regulating TMPRSS3 expression. Int J Mol Med 45:1851-1863 (2020). PubMed: 32186779
    • Jardim-Perassi BV  et al. RNA-Seq transcriptome analysis shows anti-tumor actions of melatonin in a breast cancer xenograft model. Sci Rep 9:966 (2019). PubMed: 30700756
    • Dan C  et al. HNF1B expression regulates ECI2 gene expression, potentially serving a role in prostate cancer progression. Oncol Lett 17:1094-1100 (2019). PubMed: 30655870
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 24 Abreviews or Q&A

    DAB with anti PLP

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    Gives good results at a dilution 1:50. Used on brain sections with an antibody against PLP

    Miss. Emily Santrau

    Verified customer

    投稿 Sep 23 2020

    DAB substrate for IHC staining

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    CNPase (ab6319) staining on murine brain tissue. Visualization step using ab64238 DAB substrate (1:50 dilution, 10min incubation time)

    Abcam user community

    Verified customer

    投稿 Jul 29 2020

    DAB substrate for IHC

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Excellent DAB substrate

    Abcam user community

    Verified customer

    投稿 May 29 2020

    A review of KLF4 staining of prostate cancer

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    This DAB substrate kit (ab94665) is easy to use, and the quality is good.


    Liyong Zhang

    Abcam user community

    Verified customer

    投稿 Aug 15 2017

    Abreview for DAB substrate kit

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    This kit is streamlined to use at ease. The results is fantastic.


    Liyong Zhang

    Abcam user community

    Verified customer

    投稿 Aug 15 2017

    IHC-P Mouse pancreas

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Ki67 IHC staining on mouse pancreas, blocking with 10% BSA, 1 hr ab incubation RT

    Abcam user community

    Verified customer

    投稿 Aug 16 2016

    Question

    Thank you for your prompt response. Do you recommend any buffer to go with the heat mediated retrieval, citrate, Tris/EDTA? DAB detection system would suit, I see you have detection kits such as https://www.abcam.com/Mouse-and-Rabbit-Specific-HRPDAB-ABC-detection-IHC-kit-ab64264.html that include secondary antibody.

    I will performing immunohistochemisty using DSG3 (3G133), p63 (4A4) and RTNK2 (2C2) and if possible would like a detection kit to suit all. Would https://www.abcam.com/Mouse-and-Rabbit-Specific-HRPDAB-ABC-detection-IHC-kit-ab64264.html be suitable for this?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2012

    Answer

    Thank you for contacting us.
    The kit will work with the rabbit and mouse primary antibodies.
    You can try citrate buffer pH for a start and Tris/EDTA in case the staining does not work. Please check the following for protocol and troubleshooting of;
    https://www.abcam.com/index.html?pageconfig=resource&rid=11384
    https://www.abcam.com/index.html?pageconfig=resource&rid=11467
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 06 2012

    Question

    I am enquiring about Rhotekin 2 primary antibody for use with IHC-P. I see you have an antibody clone 2C2, can you provide any information on appropriate epitope retrieval methods and detection kits or secondary antibody that would be suitable for use with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2012

    Answer

    Thank you for contacting us.
    Heat mediated antigen retrieval method with microwave or pressure cooker is recommended for this ab.
    The suitable detection system depends on the substrate you want to use. e.g. with DAB or AEC, we have following products;
    https://www.abcam.com/DAB-Enhancer-ab675.html
    https://www.abcam.com/DAB-Substrate-Kit-ab64238.html
    https://www.abcam.com/AEC-Substrate-System-Ready-to-Use-ab64252.html
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2012

    Question

    It is not idea of searching the items.. I told you that we need all reagents needed to do full IHC experiment based on your technical experience.. So if we need more items other than the ones who sent to me, please let me know.. Like antibosy diluent, antigen retrival .. etc, The customers is new to IHC and we are not experts in IHC as well.. So please advice..



    It is ok about the monoclonal rabies, waiting your kind e-mail !



    Truly yours,

    Omar

    Read More

    Abcam community

    Verified customer

    Asked on Aug 03 2012

    Answer

    Thank you for contacting us again.



    ab1002 Anti-Rabies Virus antibody looks very promising as it has been established for the use in IHC-P and should detect a wide range of rabies strains. As a starting dilution the customer can try a 5 ug/ml dilution incubated overnight at 4C. However, it may be that the actual optimum dilution may be lower or higher, but this depends on her samples. Another point is that I would strongly recommend a heat induced Antigen Retrieval if her slides are fixed with formalin.


    https://www.abcam.com/Rabies-Virus-antibody-1C5-ab1002.html (or use the following: https://www.abcam.com/Rabies-Virus-antibody-1C5-ab1002.html).


    A matching secondary antibody would be Goat polyclonal Secondary Antibody to Mouse IgG2a - heavy chain (Biotin), pre-adsorbed (ab98696)


    https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-Biotin-preadsorbed-ab98696.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG2a-heavy-chain-Biotin-preadsorbed-ab98696.html).


    To be fair, I am not the biggest fan of ready- to- use solution (andmost of my colleagues here a neither), for the following reason: most of all antibodies need optimization, may it be the blocking step, the retrieval time or method, the antibody incubation buffer etc. With ready-mix solutions it almost impossible to adjust the experiment settings. Ready mix solution make sense if the antibody is used in a high -throughput lab (like 20 slides at once with a fixed protocol), but not if you have to "learn" IHC-P and have the freedom to adjust the protocol fore the perfect results. In addition, it's cheaper ;)


    Of course, once the protocol is optimized, using ready mixed solution are a very time efficient and make life easier, but I wouldn't use them as a beginner.


    All the buffers your customer needs (see attachment) are listed in our very detailed and easy to follow step-by-step our protocol. However, none of my advice is written in stone, so if your customer prefers the ready mix solutions, we are more than happy to sell them to her.


    Something we cannot provide are the reagents for the de-paraffinization, I am afraid, but a company like Sigma should be able to help with that.


    Pease sent the customer our protocol and my advice. I am more than happy to assist her to get her experiments running. And another thing: she shouldn't be shy to ask any question she may have about the protocol!

    I hope this more information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 03 2012

    Question

    Hope you are doing well !

    Please see below an e-mail, we need to do complete Rabies IHC test... i.e all materials from Antibody diluent to retrival to everything..


    Please advice me ASAP.. Also the customer needs specific protocol, as she is very new to IHC.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 31 2012

    Answer

    Thank you for contacting us.

    Here is the list with the requested items. However, please be aware that this is not a technical inquiry as all item could be found by using the search application on our website under the "reagents" category. Please let us know if you need help on navigating our website so you can help your customers faster than relying on us.


    Regarding the Rabies antibody, I am afraid we do not have what your customer requests: All we have are mouse monoclonal antibodies against this virus, but none produced in goat. Please let me know if this expectable for her, so I can suggest the right primary antibody with the matching biotinylated secondary antibody.


    https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html (or use the following: https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html).

    https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html (or use the following: https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html).

    https://www.abcam.com/DAB-Substrate-Kit-ab64238.html (or use the following: https://www.abcam.com/DAB-Substrate-Kit-ab64238.html).

    https://www.abcam.com/Streptavidin-HRP-ready-to-use-ab64269.html (or use the following: https://www.abcam.com/Streptavidin-HRP-ready-to-use-ab64269.html).

    https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab94666.html (or use the following: https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab94666.html).

    https://www.abcam.com/Protein-Block-ab128988.html (or use the following: https://www.abcam.com/Protein-Block-ab128988.html).

    https://www.abcam.com/Mayers-Hematoxylin-ab128990.html (or use the following: https://www.abcam.com/Mayers-Hematoxylin-ab128990.html).



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 31 2012

    1-10 of 24 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 3
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.