D-Sorbitol Assay Kit (Colorimetric) (ab118968)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Food samples
- Sensitivity: 0.1 nmol/well
製品の概要
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製品名
D-Sorbitol Assay Kit (Colorimetric) -
検出方法
Colorimetric -
サンプルの種類
Food samples -
アッセイタイプ
Quantitative -
検出感度
> 0.1 nmol/well -
検出範囲
0.1 nmol/well - 10 nmol/well -
全工程の試験時間
0h 40m -
製品の概要
Abcam's D-Sorbitol Assay Kit (Colorimetric) is designed to measure sorbitol in a variety of samples such as foods, fruits, fruit juices, pharmaceuticals, cosmetics, paper. This assay is not recommended for plasma, serum or urine samples. In the assay, sorbitol is oxidized to fructose with the proportional development of intense color with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-10 nmol of Sorbitol per sample.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K631 D-Sorbitol Colorimetric Assay Kit. K631-100 is the same size as the 100 test size of ab118968.
Sorbitol is one of the 6 carbon sugar alcohols. It is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. Sorbitol is produced naturally in a variety of fruits. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can be trapped in cells and is believed to be one of the causes of damage (due to osmotic effects) in diabetes. Interestingly, sorbitol can be used as a screen for the O154:H7 strain of E. coli, since this strain is one of the few strains which cannot metabolize sorbitol.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Sorbitol Assay Buffer 1 x 25ml Sorbitol Probe 1 x 200µl Sorbitol Developer 1 vial Sorbitol Enzyme Mix 1 vial Sorbitol Standard 1 x 100µl -
研究分野
画像
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D-Sorbitol measured in various samples showing nmol/mg (fruits) and nmol/mL (juice). 300 mg (wet weight) of prune and pear was homogenised in 1 mL of water. Juice was diluted in water. Samples were diluted 2-1000 fold.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Example of standard curve obtained using ab118968.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab118968 は 7 報の論文で使用されています。
- Parekh M et al. Microbioreactor for lower cost and faster optimisation of protein production. Analyst 145:6148-6161 (2020). PubMed: 32869772
- Bearham J et al. Effective glucose metabolism maintains low intracellular glucose in airway epithelial cells after exposure to hyperglycemia. Am J Physiol Cell Physiol 317:C983-C992 (2019). PubMed: 31433692
- Maresch CC et al. Hyperglycemia induces spermatogenic disruption via major pathways of diabetes pathogenesis. Sci Rep 9:13074 (2019). PubMed: 31506549
- Song ??? Z et al. Role of fructose and fructokinase in acute dehydration-induced vasopressin gene expression and secretion in mice. J Neurophysiol 117:646-654 (2017). PubMed: 27852737
- Andres-Hernando A et al. Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice. Nat Commun 8:14181 (2017). PubMed: 28194018
- Ruiz de Azua I et al. Adipocyte cannabinoid receptor CB1 regulates energy homeostasis and alternatively activated macrophages. J Clin Invest 127:4148-4162 (2017). PubMed: 29035280
- Lanaspa MA et al. Endogenous fructose production and fructokinase activation mediate renal injury in diabetic nephropathy. J Am Soc Nephrol 25:2526-38 (2014). PubMed: 24876114