Anti-Cytoplasmic Dynein Intermediate chain 抗体 [74.1] (ab23905)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [74.1] to Cytoplasmic Dynein Intermediate chain
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1]
Cytoplasmic Dynein Intermediate chain 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [74.1] to Cytoplasmic Dynein Intermediate chain -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, IP, WBmore details
適用なし: ICC -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length native protein (purified) corresponding to Cow Cytoplasmic Dynein Intermediate chain.
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ポジティブ・コントロール
- WB: U-87 MG, SH-SY5Y, MDA-MB-231, PC-12, C6 and RAW264.7 whole cell lysates. Flow Cyt: HeLa and C6 cells. IP: U-87 MG whole cell lysate. Rat brain tissue lysate.
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特記事項
This product has switched from a hybridoma to recombinant production method on 8th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
74.1 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23905の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
1/1000.
|
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IP | (1) |
1/30.
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WB | (5) |
1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
|
特記事項 |
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Flow Cyt
1/1000. |
IP
1/30. |
WB
1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
ターゲット情報
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機能
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores. -
配列類似性
Belongs to the dynein intermediate chain family.
Contains 7 WD repeats. -
細胞内局在
Cytoplasm. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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参照データベース
- Entrez Gene: 100127187 Human
- Entrez Gene: 1780 Human
- SwissProt: O14576 Human
- SwissProt: O88485 Mouse
- SwissProt: Q63100 Rat
- Unigene: 440364 Human
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別名
- Cytoplasmic dynein 1 intermediate chain 1 antibody
- Cytoplasmic dynein intermediate chain 1 antibody
- cytosolic antibody
see all
画像
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All lanes : Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaBlocking buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-3: 37 seconds
Lanes 4-6: 81 seconds -
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).
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Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 µg with ab23905 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.
Lane 1: Rat brain tissue lysate 10 µg
Lane 2: ab23905 IP in Rat brain tissue lysate
Lane 3: Mouse monoclonal IgG instead of ab23905 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
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Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 µg with ab23905 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.
Lane 1: U-87 MG whole cell lysate 10 µg
Lane 2: ab23905 IP in U-87 MG whole cell lysate
Lane 3: Mouse monoclonal IgG instead of ab23905 in U-87 MG whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
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Flow Cytometry analysis of C6 (rat glial tumor cell line) cells labeling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 was used as the secondary antibody (red). Mouse monoclonal IgG was used as the isotype control (black). Cells without incubation with primary and secondary antibodies were used as the unlabeled control (blue).
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Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution + Rat Sciatic nerve whole tissue lysate at 60 µg
Secondary
HRP-conjugated Goat anti-mouse IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDaGel: 10% acryl amid
Blocking Step: 5% Milk for 30 minutes at 25°CThis image was generated from the hybridoma version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab23905 は 35 報の論文で使用されています。
- Shrestha J et al. HIV-1 gp120 Impairs Spatial Memory Through Cyclic AMP Response Element-Binding Protein. Front Aging Neurosci 14:811481 (2022). PubMed: 35615594
- Resnik N et al. Molecular, morphological and functional properties of tunnelling nanotubes between normal and cancer urothelial cells: New insights from the in vitro model mimicking the situation after surgical removal of the urothelial tumor. Front Cell Dev Biol 10:934684 (2022). PubMed: 36601539
- van der Horst J et al. Dynein regulates Kv7.4 channel trafficking from the cell membrane. J Gen Physiol 153:N/A (2021). PubMed: 33533890
- Jing Z et al. Rotavirus Viroplasm Biogenesis Involves Microtubule-Based Dynein Transport Mediated by an Interaction between NSP2 and Dynein Intermediate Chain. J Virol 95:e0124621 (2021). PubMed: 34379449
- Kumari A et al. Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions. J Cell Biol 220:N/A (2021). PubMed: 34709360