Application
Western blot
Sample
Human Cell lysate - whole cell (AD169-infected HFFF)
Loading amount
100000 cells
Specification
AD169-infected HFFF
Treatment
mock/endoH/PNGaseF for 16H
Gel Running Conditions
Reduced Denaturing
Blocking step
Superblock Buffer (Pierce) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Dilution
1/4000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST 10% Superblock buffer (Pierce)
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Supersignal Westpico Chemiluminescent su
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 150, 52 mock treated kDa
Additional data
Additional Notes
1: mock treated
2: Endo H digested
3: PNGase F digested
NP40 lysis buffer
2: Endo H digested
3: PNGase F digested
NP40 lysis buffer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 12 2008