Anti-Cytomegalovirus 抗体 [6F12] (ab6500)

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Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)
  • ELISA - Anti-Cytomegalovirus antibody [6F12] (ab6500)
  • Western blot - Anti-Cytomegalovirus antibody [6F12] (ab6500)

Key features and details

  • Mouse monoclonal [6F12] to Cytomegalovirus
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytomegalovirus antibody [6F12]
    Cytomegalovirus 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [6F12] to Cytomegalovirus

  • 由来種

    Mouse

  • 特異性

    Target Protein: Cytomegalovirus ICP22 Delayed Early Protein, also known as HWLF1.

  • アプリケーション

    適用あり: WB, ELISA, ICC/IFmore details

  • 種交差性

    交差種: Other species

  • 免疫原

    Cytomegalovirus, CMV

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    6F12

  • ミエローマ

    NS1/1-Ag4-1

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/800.
ELISA Use at an assay dependent concentration.
ICC/IF 1/800.

ターゲット情報

  • 関連性

    Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period.

  • 別名

    • 65 kDa phosphoprotein antibody
    • Envelope glycoprotein B antibody
    • Human herpesvirus 5 antibody
    • Phosphoprotein UL83 antibody
    • UL83 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)

    See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)

    See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytomegalovirus antibody [6F12] (ab6500)

    Determined by IFA (see protocol) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain

  • ELISA - Anti-Cytomegalovirus antibody [6F12] (ab6500)
    ELISA - Anti-Cytomegalovirus antibody [6F12] (ab6500)

    See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.

  • Western blot - Anti-Cytomegalovirus antibody [6F12] (ab6500)
    Western blot - Anti-Cytomegalovirus antibody [6F12] (ab6500)

    See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

    See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

参考文献 (1)

ab6500 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6500 は 1 報の論文で使用されています。

  • Fukui Y  et al. Establishment of a cell-based assay for screening of compounds inhibiting very early events in the cytomegalovirus replication cycle and characterization of a compound identified using the assay. Antimicrob Agents Chemother 52:2420-7 (2008). PubMed: 18458124

レビューと Q&A

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Question

we are considering the usefulness of your ab6500 antibody in some of our experiments and clinical routine. However, for a better evaluation of the appropriateness of your reagent, it would be very helpful to know how the CMV-infected cell extract you are using as an antigen has been prepared, and how the protein amount is calculated. We also need to know what kind of cells have been used (I guess fibroblast) and the strain, but above all the method you used for preparing the extract itself. The reference would be perfect. Thanking you in advance for your help I send you my best regards

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Answer

Thank you for your enquiry and patience. The originator of this antibody has been able to provide me with the following information. Virus Strain = AD169 Host Cell = Normal Human Dermal Fibroblast (NHDF)Cell line also known as HFS. Protein is determined using a BCA reagent and BSA as the protein standard. Procedure for making the extract is not available. If you have any additional questions, please contact us again.

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