Cytokine Array - Rat Cytokine 抗体 Array (Membrane, 34 Targets) (ab133992)
製品の概要
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製品名
Cytokine Array - Rat Cytokine Antibody Array (Membrane, 34 Targets)
Cytokine Antibody Array antibody arrays 製品一覧 -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Semi-quantitative -
種交差性
交差種: Rat -
製品の概要
ab133992 is for simultaneous detection of 34 Rat Cytokines. Suitable for all sample types.
Targets: Activin A, Agrin, Thymus Chemokine-1, B7-2/CD86, CINC-1, CINC-2alpha, CINC-3, CNTF, Fas Ligand, Fractalkine, GM-CSF, ICAM-1, IFN-gamma, IL-1 R6, IL-10, IL-13, IL-1alpha, IL-1beta, IL-2, IL-4, IL-6, Leptin, LIX, L-Selectin, MCP-1, MIP-3alpha, MMP-8, beta-NGF, PDGF-AA, Prolactin R, RAGE, TIMP-1, TNF-alpha, VEGF.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
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特記事項
If you are interested in this cytokine array, a table listing all of our rat membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials Cytokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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特記事項 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab133992 は 7 報の論文で使用されています。
- Matyśniak D et al. P2X7 receptor: the regulator of glioma tumor development and survival. Purinergic Signal 18:135-154 (2022). PubMed: 34964926
- Kung WM et al. Anti-Inflammatory CDGSH Iron-Sulfur Domain 2: A Biomarker of Central Nervous System Insult in Cellular, Animal Models and Patients. Biomedicines 10:N/A (2022). PubMed: 35453528
- O'Hare M et al. Loss of TRPV2-mediated blood flow autoregulation recapitulates diabetic retinopathy in rats. JCI Insight 7:N/A (2022). PubMed: 36134661
- Guzmán-Navarro G et al. Prenatal indole-3-carbinol administration activates aryl hydrocarbon receptor-responsive genes and attenuates lung injury in a bronchopulmonary dysplasia model. Exp Biol Med (Maywood) 246:695-706 (2021). PubMed: 33148012
- Fathi E et al. Cytokines secreted from bone marrow derived mesenchymal stem cells promote apoptosis and change cell cycle distribution of K562 cell line as clinical agent in cell transplantation. PLoS One 14:e0215678 (2019). PubMed: 31009502
- Qiao Z et al. Fermentation Products of Paenibacillus bovis sp. nov. BD3526 Alleviates the Symptoms of Type 2 Diabetes Mellitus in GK Rats. Front Microbiol 9:3292 (2018). PubMed: 30687277
- Mazzio EA et al. Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2- in LPS/IFN? activated glioma cells. J Neuroimmunol 302:10-19 (2017). PubMed: 27956075