Anti-Cytokeratin 8+18 抗体 [5D3], prediluted (ab847)

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Key features and details

  • Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytokeratin 8+18 antibody [5D3], prediluted
    Cytokeratin 8+18 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted

  • 由来種

    Mouse

  • 特異性

    This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Human breast carcinoma cell line MCF-7.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • CARD2 antibody
      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK18 antibody
      • CK8 antibody
      • CYK18 antibody
      • CYK8 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin 8 antibody
      • Cytokeratin-8 antibody
      • K18 antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 18 antibody
      • Keratin 8 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 18 antibody
      • keratin type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • KRT18 antibody
      • KRT8 antibody
      • type II cytoskeletal 8 antibody
      • Type-II keratin Kb8 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded human skin tissue sections, labelling Cytokeratin 8/18 with ab847.

    参考文献 (0)

    ab847 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab847 は論文での使用が確認できていません。

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