Anti-Cytokeratin 8+18 抗体 [5D3], prediluted (ab847)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)](https://www.abcam.co.jp/ps/products/0/ab847/Images/ab847-2-anti-cytokeratin-8-18-antibody-5d37-immunohistochemistry-skin-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)")
Key features and details
- Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Cytokeratin 8+18 antibody [5D3], prediluted
Cytokeratin 8+18 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted -
由来種
Mouse -
特異性
This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). This antibody stains simple and glandular epithelium. This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Cytokeratin 8+18. Human breast carcinoma cell line MCF-7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
5D3 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab847の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. |
| 特記事項 |
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IHC-P
Use at an assay dependent concentration. This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. |
ターゲット情報
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関連性
Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3856 Human
- Entrez Gene: 3875 Human
- Omim: 148060 Human
- Omim: 148070 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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別名
- CARD2 antibody
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)Formalin-fixed, paraffin-embeded human skin tissue stained for Cytokeratin 8 +18 using ab847 in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)](https://www.abcam.co.jp/ps/products/0/ab847/Images/ab847-255298-anti-cytokeratin-818-antibody-5d3-prediluted-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3], prediluted (ab847)Immunohistochemical analysis of formalin-fixed paraffin-embedded human skin tissue sections, labelling Cytokeratin 8/18 with ab847.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab847 は論文での使用が確認できていません。
