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Anti-Cytokeratin 8+18 抗体 [5D3] (ab17139)

Reviews (1)Q&A (1)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
  • Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

Key features and details

  • Mouse monoclonal [5D3] to Cytokeratin 8+18
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Cytokeratin 8+18 antibody [5D3]
    Cytokeratin 8+18 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5D3] to Cytokeratin 8+18

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Cytokeratin 8+18. Cytokeratins from the human breast carcinoma cell line MCF-7

  • ポジティブ･コントロール

    • IHC-P: Human Skin tissue. Flow cyt: MCF7 cells.

  • 特記事項


    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • 一次抗体 備考

    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

  • ポリ/モノ

    モノクローナル

  • クローン名

    5D3

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17139の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration. PubMed: 21102523
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
特記事項
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 21102523
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 関連性

    Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • CARD2 antibody
      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK18 antibody
      • CK8 antibody
      • CYK18 antibody
      • CYK8 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin 8 antibody
      • Cytokeratin-8 antibody
      • K18 antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 18 antibody
      • Keratin 8 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 18 antibody
      • keratin type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • KRT18 antibody
      • KRT8 antibody
      • type II cytoskeletal 8 antibody
      • Type-II keratin Kb8 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Paraffin embedded human skin tissue stained for Cytokeratin 8/18 using ab 17139 in immunohistochemical analysis.
    • Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
      Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

      Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    参考文献 (5)

    ab17139 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17139 は 5 報の論文で使用されています。

    • Yang BY  et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-?B pathway inhibition. Acta Biomater 88:392-405 (2019). PubMed: 30753941
    • Bhat V  et al. Isolation and characterization of a new basal-like luminal progenitor in human breast tissue. Stem Cell Res Ther 10:269 (2019). PubMed: 31443683
    • Ramachandra Rao S  et al. Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome. Autophagy 14:1796-1817 (2018). PubMed: 29979914
    • Xu D  et al. Enabling autologous human liver regeneration with differentiated adipocyte stem cells. Cell Transplant 23:1573-84 (2014). PubMed: 24148223
    • Wang Y  et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . PubMed: 21102523

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 8+18 antibody [5D3]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF7 Breast Cancer Cells)
    Specification
    MCF7 Breast Cancer Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    Dr. Sharon Sneddon

    Verified customer

    投稿 Nov 30 2011

    Question

    Interested in one of your keratin 8 +18 antibodies. I'd like to use it on human formalin fixed parafin embedded (FFPE) liver samples that are 5 microns thick. Which clone would you recommend for this as there are several different available? Thanks,

    Read More
    Answer

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Cytokeratin 8+18 antibody- antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    Taking this into consideration, my recommendations would be ab17139 or ab15224.

    https://www.abcam.com/Cytokeratin-8-18-antibody-5D3-ab17139.html

    https://www.abcam.com/Cytokeratin-8-18-antibody-SP141-ab15224.html



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com