Alexa Fluor® 488 Anti-Cytokeratin 8 抗体 [EP1628Y] (ab192467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP1628Y] to Cytokeratin 8
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
Cytokeratin 8 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EP1628Y] to Cytokeratin 8 -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human Cytokeratin 8 aa 300-400 (C terminal). The exact sequence is proprietary.
Database link: P05787 -
ポジティブ・コントロール
- ICC/IF: HeLa cells Flow Cyt (intra): HeLa cells
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
解離定数(KD 値)
KD = 4.60 x 10 -10 M Learn more about KD -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1628Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
- HRP Anti-Cytokeratin 8 antibody [EP1628Y] (ab193094)
- PE Anti-Cytokeratin 8 antibody [EP1628Y] (ab209297)
- Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (ab210139)
- Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free (ab217173)
- Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
1/100.
|
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Flow Cyt (Intra) |
1/500.
|
特記事項 |
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ICC
1/100. |
Flow Cyt (Intra)
1/500. |
ターゲット情報
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機能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
組織特異性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
関連疾患
Cirrhosis -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
細胞内局在
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- Unigene: 533782 Human
- Unigene: 708445 Human
- Unigene: 358618 Mouse
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別名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
画像
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Immunofluorescence analysis of mouse myoepithelial cells labelling KRT8 (right) with ab192467 at 1/100 dilution. The tissue was fixed in 10% neutral buffered formalin overnight. Paraffin embedding and sectioning were performed by the Rodent Histopathology Core at Harvard Medical School. Scale bar, 10 μm.
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Immunofluorescence analysis of mouse postate tumor samples labelling cytokeratin 8 (green) with ab192467 at 1/100 dilution. SYP was also stained using ab206870 (red). Cells were fixed with formalin and embedded in paraffin. Sections were blocked with PBS-Tween (0.1%) containing 5% of BSA. Primary conjugated antibodies were simultaneously incubated overnight at 4°C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 µm.
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Immunofluorescence staining of cytokeratin 8 in HeLa cells using ab192467. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192647 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) (ab150120) at 2 μg/ml (shown in pseudo-color red).
Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)
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Flow cytometry analysis of HeLa cells stained with ab192467. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192467, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunofluorescence staining in human mammary organoids of cytokeratin 8 using ab192467 (green), cytokeratin 14 using ab206100 (red), and actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 µm. Organoids were imaged by confocal microscopy.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab192467 は 12 報の論文で使用されています。
- Johnson BE et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med 3:100525 (2022). PubMed: 35243422
- Burlingame EA et al. Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms. Cell Rep Methods 1:N/A (2021). PubMed: 34485971
- Mohad V et al. Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis. Dev Biol 473:33-49 (2021). PubMed: 33515576
- Singh S et al. Pan-cancer Drivers are Recurrent Transcriptional Regulatory Heterogeneities in Early-stage Luminal Breast Cancer. Cancer Res N/A:N/A (2021). PubMed: 33531373
- Sulsenti R et al. Repurposing of the Antiepileptic Drug Levetiracetam to Restrain Neuroendocrine Prostate Cancer and Inhibit Mast Cell Support to Adenocarcinoma. Front Immunol 12:622001 (2021). PubMed: 33737929