Anti-Cytokeratin 8 抗体 [35bH11] (ab758)
製品の概要
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製品名Anti-Cytokeratin 8 antibody [35bH11]
Cytokeratin 8 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [35bH11] to Cytokeratin 8
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由来種Mouse
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アプリケーション適用あり: IHC-P, IHC-Frmore details
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種交差性交差種: Human
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免疫原
Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名35bH11
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アイソタイプIgM
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軽鎖の種類unknown
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | ||
| IHC-Fr |
追加情報
IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
ターゲット情報
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機能Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
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組織特異性Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
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関連疾患Cirrhosis
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配列類似性Belongs to the intermediate filament family.
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翻訳後修飾Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
細胞内局在Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 3856 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
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別名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)](https://www.abcam.co.jp/ps/products/0/ab758/Images/ab758_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)ab758 - immunohistochemistry
Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)](https://www.abcam.co.jp/ps/products/0/ab758/Images/Cytokeratin-8-Primary-antibodies-ab758-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.
参考文献
This product has been referenced in:
- Berger R et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"