Anti-Cytokeratin 8 抗体 [35bH11] (ab758)

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製品の概要

  • 製品名
    Anti-Cytokeratin 8 antibody [35bH11]
    Cytokeratin 8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [35bH11] to Cytokeratin 8
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
  • 追加情報
    IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.

    • ターゲット情報

    • 機能
      Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • 組織特異性
      Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • 関連疾患
      Cirrhosis
    • 配列類似性
      Belongs to the intermediate filament family.
    • 翻訳後修飾
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • 細胞内局在
      Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • 参照データベース
      • 別名
        • CARD2 antibody
        • CK 8 antibody
        • CK-8 antibody
        • CK8 antibody
        • CYK8 antibody
        • CYKER antibody
        • Cytokeratin endo A antibody
        • Cytokeratin-8 antibody
        • DreK8 antibody
        • EndoA antibody
        • K2C8 antibody
        • K2C8_HUMAN antibody
        • K8 antibody
        • Keratin 8 antibody
        • Keratin type II cytoskeletal 8 antibody
        • Keratin, type II cytoskeletal 8 antibody
        • Keratin-8 antibody
        • KO antibody
        • Krt 2.8 antibody
        • KRT8 antibody
        • MGC118110 antibody
        • MGC174782 antibody
        • MGC53564 antibody
        • MGC85764 antibody
        • sb:cb186 antibody
        • Type-II keratin Kb8 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)

        ab758 - immunohistochemistry

        Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [35bH11] (ab758)
        ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.

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      参考文献

      This product has been referenced in:
      • Berger R  et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »
      See 1 Publication for this product

      ab758 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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      Question


      Inquiry: I'm looking into a panel of antibodies from your company to use in cells of pig origin. ab758 ab27988 ab7504 ab124687 ab28028 ab11333 ab32362 I would like to know the following: Have they been tested in pig cells? If yes, are they reactive towards pig cells? If you do not have that information, can you tell me the epitope or peptide sequence that was used in generating that antibody? If you do not want to tell me that info, can you tell me the identity percentage of match of the pig compared to the epitope that it was used in generating the antibody. Many thanks! S

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      Answer

      Thank you for your inquiry.

      For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig cytokeratin 8 sequence. We generally say anything with >85% homology would be expected to cross-react, so this is right on the edge. However, we do have two anti-cytokeratin 8 antibodies that we know do work in pig as ab2531 and ab2530.

      https://www.abcam.com/Cytokeratin-8-antibody-C-51-ab2531.html

      https://www.abcam.com/Cytokeratin-8-antibody-C-43-ab2530.html

      For ab27988, this antibody has been validated in pig and we therefore guarantee it will work for pig in IHC-P, IHC-Fr in accordance with our Abpromise.

      For ab7504, the immunogen was human breast carcinoma cell line, MCF-7. Pig and human EpCAM is 83% homologous, so this is a little low. We have an anti-EpCAM that is predicted (>90% homology) to cross-react with pig, and that is ab71916. However, this hasn't been validated and therefore not guaranteed in pig.

      https://www.abcam.com/EpCAM-antibody-ab71916.html

      I have to check with the lab on ab32362 and ab124687 since I don't have the immunogens readily available to BLAST.

      For ab28028, full length native protein (purified): Vimentin isolated from bovine eye lens was used as an immunogen. Pig vimentin and bovine vimentin are 97% homologous, so there is a good chance this will cross-react with pig. However this hasn't been tested and therefore isn't validated in pig. We do have a few vimentin antibodies that work in pig though,like ab20346 and ab8069.

      https://www.abcam.com/Vimentin-antibody-VI-10-ab20346.html

      https://www.abcam.com/Vimentin-antibody-V9-ab8069.html

      For ab11333, cultured human thymic fibroblasts was used as an immunogen. We don't have a particular protein sequence to look up for this one, so unfortunately we don't know how/if it will work with pig.

      I hope this information helps. I'll be in touch again once I hear about ab32362 and ab124687.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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