Anti-Cytokeratin 7+17 抗体 [C-46] (ab670)
製品の概要
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製品名Anti-Cytokeratin 7+17 antibody [C-46]
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製品の詳細Mouse monoclonal [C-46] to Cytokeratin 7+17
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由来種Mouse
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特異性Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa)
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アプリケーション適用あり: ICC, WB, IHC-P, IPmore details
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種交差性交差種: Sheep, Cow, Dog, Human, Pig
非交差種: Mouse, Rat, Rabbit -
免疫原
Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).
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特記事項Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度>95% by SDS-PAGE
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特記事項（精製）Purified from culture supernatant using protein A-affinity chromatography.
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ポリ/モノモノクローナル
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クローン名C-46
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | ||
| WB | ||
| IHC-P | ||
| IP |
追加情報
ICC: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependant dilution.
WB: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependant dilution.
WB: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling.
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細胞内局在Cytoplasmic
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参照データベース
- Entrez Gene: 3855 Human
- Entrez Gene: 3872 Human
- SwissProt: P08729 Human
- SwissProt: Q04695 Human
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別名
- CK17 antibody
- CK7 antibody
- Cytokeratin 17 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/Cytokeratin-717-Primary-antibodies-ab670-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue. -
![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/Cytokeratin-717-Primary-antibodies-ab670-2.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate
Observed band size: 49,51 kDa why is the actual band size different from the predicted?
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-287516-ab670.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human breast by immunohistochemistry using formalin fixed, paraffin embedded tissue.
プロトコール
参考文献
This product has been referenced in:
- Sun Y et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). Read more (PubMed: 19524636) »
- Vojtesek B et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). Read more (PubMed: 1695327) »
