Anti-Cytokeratin 7+17 抗体 [C-46] (ab670)

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Key features and details

  • Mouse monoclonal [C-46] to Cytokeratin 7+17
  • Suitable for: WB, IHC-P
  • Reacts with: Sheep, Cow, Dog, Human, Pig
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytokeratin 7+17 antibody [C-46]

  • 製品の詳細

    Mouse monoclonal [C-46] to Cytokeratin 7+17

  • 由来種

    Mouse

  • 特異性

    Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa)

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Sheep, Cow, Dog, Human, Pig
    非交差種: Mouse, Rat, Rabbit

  • 免疫原

    Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).

  • 特記事項

    Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Purified from culture supernatant. Purity >95% by SDS-PAGE.

  • ポリ/モノ

    モノクローナル

  • クローン名

    C-46

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • CK17 antibody
      • CK7 antibody
      • Cytokeratin 17 antibody
      • Cytokeratin 7 antibody
      • K17 antibody
      • K7 antibody
      • Keratin 17 antibody
      • Keratin 7 antibody
      • KRT17 antibody
      • KRT7 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
      Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
    • Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
      Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
      Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate

      Observed band size: 49,51 kDa
      why is the actual band size different from the predicted?



      Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)

      Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human breast by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    参考文献 (2)

    ab670 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab670 は 2 報の論文で使用されています。

    • Sun Y  et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). PubMed: 19524636
    • Vojtesek B  et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). PubMed: 1695327

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 7+17 antibody [C-46]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Nasal epithelium)
    Permeabilization
    No
    Specification
    Nasal epithelium
    Fixative
    heat (67C, 15 min)
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 10 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 7+17 antibody [C-46]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Cow Tissue sections (Tongue)
    Permeabilization
    No
    Specification
    Tongue
    Fixative
    heat (67C, 15 min)
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 11 2018

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