Key features and details
- Mouse monoclonal [C-46] to Cytokeratin 7+17
- Suitable for: WB, IHC-P
- Reacts with: Sheep, Cow, Dog, Human, Pig
- Isotype: IgG1
製品名Anti-Cytokeratin 7+17 antibody [C-46]
製品の詳細Mouse monoclonal [C-46] to Cytokeratin 7+17
特異性Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa)
アプリケーション適用あり: WB, IHC-Pmore details
種交差性交差種: Sheep, Cow, Dog, Human, Pig
非交差種: Mouse, Rat, Rabbit
Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).
特記事項Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
精製度Protein A purified
特記事項（精製）Purified from culture supernatant. Purity >95% by SDS-PAGE.
Our Abpromise guarantee covers the use of ab670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
関連性Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling.
- CK17 antibody
- CK7 antibody
- Cytokeratin 17 antibody
Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate
Observed band size: 49,51 kDa why is the actual band size different from the predicted?
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human breast by immunohistochemistry using formalin fixed, paraffin embedded tissue.
ab670 は 2 報の論文で使用されています。
- Sun Y et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). PubMed: 19524636
- Vojtesek B et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). PubMed: 1695327