Anti-Cytokeratin 7+17 抗体 [C-46] (ab670)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-3606-anti-cytokeratin-717-antibody-c-46-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)")
Key features and details
- Mouse monoclonal [C-46] to Cytokeratin 7+17
- Suitable for: WB, IHC-P
- Reacts with: Sheep, Cow, Dog, Human, Pig
- Isotype: IgG1
製品の概要
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製品名
Anti-Cytokeratin 7+17 antibody [C-46] -
製品の詳細
Mouse monoclonal [C-46] to Cytokeratin 7+17 -
由来種
Mouse -
特異性
Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa) -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Sheep, Cow, Dog, Human, Pig
非交差種: Mouse, Rat, Rabbit -
免疫原
Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).
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特記事項
Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
C-46 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3855 Human
- Entrez Gene: 3872 Human
- SwissProt: P08729 Human
- SwissProt: Q04695 Human
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別名
- CK17 antibody
- CK7 antibody
- Cytokeratin 17 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-3607-anti-cytokeratin-717-antibody-c-46-western-blot.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate
Observed band size: 49,51 kDa why is the actual band size different from the predicted?
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-287516-anti-cytokeratin-717-antibody-c-46-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human breast by immunohistochemistry using formalin fixed, paraffin embedded tissue.
プロトコール
参考文献 (2)
ab670 は 2 報の論文で使用されています。
- Sun Y et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). PubMed: 19524636
- Vojtesek B et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). PubMed: 1695327
