Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (thyroid follicular tumor, follicular cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
thyroid follicular tumor, follicular cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/800
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Species: Horse
Dilution: 1:800
Pretreatment for antigen retrieval: microwave heating with citrate buffer for 12 minutes at 600 watts
Results: positive
Secondary antibody: GAMouse-b 1:200 (diluted in PBS/Goat normal Serum)
stain reagent: DAB for 8 minutes at room temperature
Counter stain: Hämalaun for 2 minutes at room temperature
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 17 2017