Application
Immunocytochemistry
Sample
Human Cultured Cells (Primary breast)
Specification
Primary breast
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/25
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS + 10% goat serum
Secondary antibody
Name
Non-Abcam antibody was used: UltraVision ONE HRP Polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Image: Breast organoids derived from normal breast tissue showing staining of various epithelial cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Rene Villadsen
Verified customer
投稿 Jun 27 2011