Anti-Cytokeratin 6 抗体 [Ks6.KA12] (ab18586)
![Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586)](https://www.abcam.co.jp/ps/products/18/ab18586/Images/ab18586-28219-anti-cytokeratin-6-antibody-ks6ka12-flow-cytometry.jpg "Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586)")
Key features and details
- Mouse monoclonal [Ks6.KA12] to Cytokeratin 6
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Cytokeratin 6 antibody [Ks6.KA12]
Cytokeratin 6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Ks6.KA12] to Cytokeratin 6 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
A cytoskeletal preparation of human callus material was used for immunization.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified by protein A affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
Ks6.KA12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18586の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
|
Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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組織特異性
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 286887 Human
- Entrez Gene: 3853 Human
- Entrez Gene: 3854 Human
- Omim: 148041 Human
- Omim: 148042 Human
- Omim: 612315 Human
- SwissProt: P02538 Human
- SwissProt: P04259 Human
see all -
別名
- CK 6A antibody
- CK 6B antibody
- CK 6C antibody
see all
画像
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Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586)
Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab18586 は 7 報の論文で使用されています。
- Irrera N et al. PDRN, a Bioactive Natural Compound, Ameliorates Imiquimod-Induced Psoriasis through NF-?B Pathway Inhibition and Wnt/ß-Catenin Signaling Modulation. Int J Mol Sci 21:N/A (2020). PubMed: 32059361
- Mancino G et al. The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation. Thyroid 30:1066-1078 (2020). PubMed: 32111151
- Medgyesi B et al. Rosacea Is Characterized by a Profoundly Diminished Skin Barrier. J Invest Dermatol 140:1938-1950.e5 (2020). PubMed: 32199994
- Chen C & Shan H Keratin 6A gene silencing suppresses cell invasion and metastasis of nasopharyngeal carcinoma via the ß-catenin cascade. Mol Med Rep 19:3477-3484 (2019). PubMed: 30896882
- Xie HT et al. Biomarkers for Progenitor and Differentiated Epithelial Cells in the Human Meibomian Gland. Stem Cells Transl Med 7:887-892 (2018). PubMed: 30251359
- Wagner VP et al. Histogenesis of keratoacanthoma: histochemical and immunohistochemical study. Oral Surg Oral Med Oral Pathol Oral Radiol 119:310-7 (2015). IHC-P ; Human . PubMed: 25488010
- Holt BM et al. Pig dorsum model for examining impaired wound healing at the skin-implant interface of percutaneous devices. J Mater Sci Mater Med 24:2181-93 (2013). PubMed: 23832453