Anti-Cytokeratin 5 抗体 [EP1601Y] - Cytoskeleton Marker (ab52635)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1601Y] to Cytokeratin 5 - Cytoskeleton Marker
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker
Cytokeratin 5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1601Y] to Cytokeratin 5 - Cytoskeleton Marker -
由来種
Rabbit -
特異性
Mouse reactivity is based on IHC (positive tissues: Liver, lung, brain and skin). However, WB was negative for Mouse brain, heart, kidney and spleen. There is background staining in mouse and rat islet.
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アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A431 cell, human fetal skin, rat skin and mouse skin lysates. IHC-P: Squamous cell cervical, squamous cell lung and basal cell breast carcinoma tissue. Human transitional urinary bladder carcinoma tissue. Normal tonsil squamous, human cervical carcinoma, mouse skin and rat skin tissues. Human normal skin tissue. Flow Cyt (intra) and ICC/IF: A431 cells. ICC/IF: A431 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1601Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y] (ab193894)
- Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] (ab193895)
- HRP Anti-Cytokeratin 5 antibody [EP1601Y] (ab193896)
- Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
- APC Anti-Cytokeratin 5 antibody [EP1601Y] (ab224984)
- PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52635の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
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ICC/IF |
1/100.
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WB | (2) |
1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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IHC-P | (4) |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/20. |
ICC/IF
1/100. |
WB
1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連疾患
Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 3852 Human
- Entrez Gene: 110308 Mouse
- Entrez Gene: 369017 Rat
- Omim: 148040 Human
- SwissProt: P13647 Human
- SwissProt: Q922U2 Mouse
- SwissProt: Q6P6Q2 Rat
- Unigene: 433845 Human
see all -
別名
- 58 kDa cytokeratin antibody
- CK-5 antibody
- CK5 antibody
see all
画像
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IHC image of Cytokeratin 5 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab52635, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
All lanes : Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution
Lane 1 : N-GST tagged full-length recombinant human Cytokeratin 6A protein,10ng
Lane 2 : N-GST tagged full-length recombinant human Cytokeratin 5 protein,10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking buffer: 5% NFDM /TBST.
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All lanes : Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/1000 dilution
Lane 1 : Human skin lysates prepared in RIPA lysis method
Lane 2 : Human skin lysates prepared in 1%SDS Hot lysis method
Lane 3 : Mouse skin lysates prepared in RIPA lysis method
Lane 4 : Mouse skin lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 62 kDaThe lysates were prepared in 1%SDS Hot lysis method.
Observed MW: 62kDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 6 with Purified ab52635 at 1/100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution (purified)
Lane 1 : Human fetal skin lysates
Lane 2 : Rat skin lysates
Lane 3 : Mouse skin lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The lysates were prepared in 1%SDS Hot lysis method.
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Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Cytokeratin 5 with purified ab52635 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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Different batches of ab52635 were tested on Rat skin lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 62 kDa.
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Colocalization of KRT5, KRT6 and KRT17 in HSC3 cells
Immunocytochemistry in HSC3 (human oral squamous carcinoma cell line) cells. Scale bar, 10 μm.
(Taken from Figure S3 of Khanom et al)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue sections labeling Cytokeratin 5 with Purified ab52635 at 1:200 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Unpurified ab52635 showing positive staining in squamous cell cervical carcinoma tissue.
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A431 cells stained with unpurified ab52635 at 1/100 - 1/250
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Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635) at 1/10000 dilution (unpurified) + A431 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa -
Human transitional urinary bladder carcinoma stained with unpurified ab52635 at 1/100 - 1/250 dilution.
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Unpurified ab52635 showing positive staining in basal cell breast carcinoma tissue.
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Unpurified ab52635 showing negative staining in ductal breast carcinoma tissue.
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Unpurified ab52635 showing negative staining in stomach adenocarcinoma tissue.
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Unpurified ab52635 showing positive staining in normal tonsil squamous cells tissue.
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Unpurified ab52635 showing positive staining in squamous cell lung carcinoma tissue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (139)
ab52635 は 139 報の論文で使用されています。
- Wang C et al. The diameter factor of aligned membranes facilitates wound healing by promoting epithelialization in an immune way. Bioact Mater 11:206-217 (2022). PubMed: 34938924
- Xu Q et al. Topical astilbin ameliorates imiquimod-induced psoriasis-like skin lesions in SKH-1 mice via suppression dendritic cell-Th17 inflammation axis. J Cell Mol Med 26:1281-1292 (2022). PubMed: 35023281
- Jhang JF et al. Improved Urothelial Cell Proliferation, Cytoskeleton and Barrier Function Protein Expression in the Patients With Interstitial Cystitis/Bladder Pain Syndrome After Intravesical Platelet-Rich Plasma Injection. Int Neurourol J 26:S57-67 (2022). PubMed: 35073671
- Morgan R et al. A medium composition containing normal resting glucose that supports differentiation of primary human airway cells. Sci Rep 12:1540 (2022). PubMed: 35087167
- van Riet S et al. Organoid-based expansion of patient-derived primary alveolar type 2 cells for establishment of alveolus epithelial Lung-Chip cultures. Am J Physiol Lung Cell Mol Physiol 322:L526-L538 (2022). PubMed: 35137633