アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    cytokeratin-5--6--18-antibody-lp34-ab40.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Cytokeratin
Share by email
リコンビナント

Anti-Cytokeratin 5 + 6 + 18 抗体 [LP34] (ab40)

  • Datasheet
Submit a review Submit a question References (12)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
  • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
  • Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
ペプチド
Cytokeratin 13 peptide (ab180923)
一次抗体
Product image
Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)

関連製品

製品の概要

  • 製品名

    Anti-Cytokeratin 5 + 6 + 18 antibody [LP34]
    Cytokeratin 5 + 6 + 18 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [LP34] to Cytokeratin 5 + 6 + 18
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • IHC-P: Human skin and tonsil tissue. Human pancreatic carcinoma and bladder carcinoma tissue. WB: Human skin lysate. HeLa, MCF7 and HepG2 whole cell lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    LP34
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Cytokeratin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

関連製品

  • Alternative Versions

    • Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] - BSA and Azide free (ab256139)
  • Related Products

    • Anti-mouse IgG for IP (HRP) (ab131368)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.424 µg/ml.
IHC-P
Use a concentration of 0.085 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.424 µg/ml.
IHC-P
Use a concentration of 0.085 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 関連性

    Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is memeber of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family.
  • 細胞内局在

    Cytoplasmic
  • 参照データベース

    • Entrez Gene: 3852 Human
    • Entrez Gene: 3853 Human
    • Entrez Gene: 3875 Human
    • Omim: 148040 Human
    • Omim: 148070 Human
    • SwissProt: P02538 Human
    • SwissProt: P05783 Human
    • SwissProt: P13647 Human
    • 別名

      • CK5 antibody
      • CYK18 antibody
      • K18 antibody
      • K5 antibody
      • K6IRS2 antibody
      • Keratin 18 antibody
      • Keratin 5 antibody
      • Keratin 72 antibody
      • KRT18 antibody
      • KRT5 antibody
      • KRT6 antibody
      • KRT72 antibody
      see all

    画像

    • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      All lanes : Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 0.424 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
      Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 15 secs; Lane 2-3: 3 secs.

    • Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Western blot - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40) at 1/1000 dilution + Human skin lysate at 20 µg

      Secondary
      Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

      Observed band size: 58 kDa why is the actual band size different from the predicted?


      Exposure time: 114 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

      Immunohistochemical analysis of paraffin-embedded human skin labeling Cytokeratin 5 + 6 + 18 with ab40 at 1/1000 dilution, followed by a ready to use secondary. Positive staining on human skin is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on the epithelial cells of human tonsil tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

      Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human bladder carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

      Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling Cytokeratin 5 + 6 + 18 with ab40 at 0.085µg/ml, followed by a ready to use secondary. Positive staining on human pancreatic carcinoma tissue is observed. The section was incubated with ab40 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)
      Anti-Cytokeratin 5 + 6 + 18 antibody [LP34] (ab40)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (12)

    ab40 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab40 は 12 報の論文で使用されています。

    • Eede P  et al. Interleukin-12/23 deficiency differentially affects pathology in male and female Alzheimer's disease-like mice. EMBO Rep 21:e48530 (2020). PubMed: 32003148
    • Haghani A  et al. Toxicity of urban air pollution particulate matter in developing and adult mouse brain: Comparison of total and filter-eluted nanoparticles. Environ Int 136:105510 (2020). PubMed: 32004873
    • Bergkvist L  et al. Mapping pathogenic processes contributing to neurodegeneration in Drosophila models of Alzheimer's disease. FEBS Open Bio 10:338-350 (2020). PubMed: 31823504
    • Lue LF  et al. Advance in Plasma AD Core Biomarker Development: Current Findings from Immunomagnetic Reduction-Based SQUID Technology. Neurol Ther 8:95-111 (2019). PubMed: 31833027
    • Tejera D  et al. Systemic inflammation impairs microglial Aß clearance through NLRP3 inflammasome. EMBO J 38:e101064 (2019). PubMed: 31359456
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab40.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.