Anti-Cytokeratin 4 抗体 [EPR1598] (ab92465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1598] to Cytokeratin 4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytokeratin 4 antibody [EPR1598]
Cytokeratin 4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1598] to Cytokeratin 4 -
由来種
Rabbit -
特異性
The immunogen shares high homology with other cytokeratins like cytokeratin-3(93%), cytokeratin-79(85.7%) and cytokeratin-2 oral(85.7%) etc. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human Cytokeratin 4.
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ポジティブ・コントロール
- WB: A431 and Human uterus cell lysates IHC-P: Human tonsil tissue ICC/IF: A431 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR1598 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IHC-P | ||
ICC/IF |
特記事項 |
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IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB: 1/1000 - 1/10,000. Predicted molecular weight: 57 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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組織特異性
Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. -
関連疾患
Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 3851 Human
- Entrez Gene: 315323 Rat
- Omim: 123940 Human
- SwissProt: P19013 Human
- SwissProt: Q6IG00 Rat
- Unigene: 654610 Human
- Unigene: 706527 Human
- Unigene: 100688 Rat
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製品の状態
Localisation: Intermediate filament (Cytoskeleton). -
別名
- CK 4 antibody
- CK-4 antibody
- CK4 antibody
see all
画像
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ab92465, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-Cytokeratin 4 antibody [EPR1598] (ab92465) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human uterus cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa -
Immunofluorescent staining of A431 cells, using ab92465 at a 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab92465 は 2 報の論文で使用されています。
- Jerg A et al. Modulation of gingival cell response towards dental composites. Dent Mater 34:412-426 (2018). PubMed: 29306486
- Jacot TA et al. Development of a composite measure of product adherence, protocol compliance, and semen exposure using DNA and protein biomarkers for topical HIV prevention studies. PLoS One 9:e114368 (2014). ICC ; Human . PubMed: 25489736