Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin (Merkel cell carcinoma))
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - 0,2% Triton/PBS
Specification
skin (Merkel cell carcinoma)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody diluent
Dilution
1/25

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: goat anti mouse Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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投稿 Feb 06 2018

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