製品の概要

  • 製品名

    Anti-Cytokeratin 20 antibody [Ks20.8]
    Cytokeratin 20 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [Ks20.8] to Cytokeratin 20
  • 由来種

    Mouse
  • 特異性

    This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas.
  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa.

  • ポジティブ・コントロール

    • Colon carcinoma. IF/ICC: Jeg3 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50.

(Also see PMID: 20332776)

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
ICC/IF 1/200.

ターゲット情報

  • 機能

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 組織特異性

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • 配列類似性

    Belongs to the intermediate filament family.
  • 発生段階

    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • 翻訳後修飾

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • 細胞内局在

    Cytoplasm.
  • Information by UniProt
  • 参照データベース

  • 別名

    • CD20 antibody
    • CK 20 antibody
    • CK-20 antibody
    • CK20 antibody
    • Cytokeratin-20 antibody
    • Cytokeratin20 antibody
    • K1C20_HUMAN antibody
    • K20 antibody
    • KA20 antibody
    • Keratin 20 antibody
    • keratin 20, type I antibody
    • keratin 21, rat, homolog of antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 20 antibody
    • Keratin-20 antibody
    • Keratin20 antibody
    • KRT 20 antibody
    • KRT 21 antibody
    • KRT20 antibody
    • KRT21 antibody
    • MGC35423 antibody
    • OTTHUMP00000164518 antibody
    • Protein IT antibody
    • type I cytoskeletal 20 antibody
    see all

画像

  • ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Immunofluorescent analysis of human colon epithelium, staining Cytokeratin 20 with ab854.

  • Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:

  • Wang Q  et al. Ketogenesis contributes to intestinal cell differentiation. Cell Death Differ 24:458-468 (2017). Read more (PubMed: 27935584) »
  • Fridriksdottir AJ  et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun 6:8786 (2015). Read more (PubMed: 26564780) »
See all 3 Publications for this product

レビューと Q&A

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin (Merkel cell carcinoma))
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - 0,2% Triton/PBS
Specification
skin (Merkel cell carcinoma)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

投稿 Feb 06 2018

Application
Western blot
Sample
Human Tissue lysate - whole (skin biopsie)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16%)
Loading amount
30 µg
Specification
skin biopsie
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

投稿 Feb 06 2018

Application
Western blot
Sample
Human Tissue lysate - whole (Colon Carcinoma)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
Colon Carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

投稿 Aug 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Organoid)
Permeabilization
Yes - 0.3% Triton
Specification
Organoid
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

投稿 Mar 10 2016

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