Anti-Cytokeratin 20 抗体 [Ks20.8] (ab854)
Key features and details
- Mouse monoclonal [Ks20.8] to Cytokeratin 20
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Cytokeratin 20 antibody [Ks20.8]
Cytokeratin 20 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Ks20.8] to Cytokeratin 20 -
由来種
Mouse -
特異性
This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas. -
アプリケーション
適用あり: Flow Cyt, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa.
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ポジティブ・コントロール
- Colon carcinoma. IF/ICC: Jeg3 cell line.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.1% Sodium azide
Constituents: PBS, Carrier protein
Van Gogh Yellow Diluent -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
Ks20.8 -
ミエローマ
unknown -
アイソタイプ
IgG2a -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | 1/50. (Also see PMID: 20332776)
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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IHC-P | Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. | |
ICC/IF | 1/200. |
ターゲット情報
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機能
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
組織特異性
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
翻訳後修飾
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 54474 Human
- Omim: 608218 Human
- SwissProt: P35900 Human
- Unigene: 84905 Human
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別名
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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Immunofluorescent analysis of human colon epithelium, staining Cytokeratin 20 with ab854.
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Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
参考文献 (3)
ab854 は 3 報の論文で使用されています。
- Wang Q et al. Ketogenesis contributes to intestinal cell differentiation. Cell Death Differ 24:458-468 (2017). PubMed: 27935584
- Fridriksdottir AJ et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun 6:8786 (2015). PubMed: 26564780
- Fang DD et al. Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery. Br J Cancer : (2010). Flow Cyt ; Human . PubMed: 20332776