Anti-Cytokeratin 20 抗体 [Ks20.8] (ab854)

Reviews (4)Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [Ks20.8] to Cytokeratin 20
  • Suitable for: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Cytokeratin 20 antibody [Ks20.8]
    Cytokeratin 20 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [Ks20.8] to Cytokeratin 20

  • 由来種

    Mouse

  • 特異性

    This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. It is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary and small cell carcinomas.

  • アプリケーション

    適用あり: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa.

  • ポジティブ･コントロール

    • Colon carcinoma. IF/ICC: Jeg3 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50.

(Also see PMID: 20332776)

 

 

 

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
ICC/IF 1/200.

ターゲット情報

  • 機能

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.

  • 組織特異性

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 発生段階

    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.

  • 翻訳後修飾

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD20 antibody
      • CK 20 antibody
      • CK-20 antibody
      • CK20 antibody
      • Cytokeratin-20 antibody
      • Cytokeratin20 antibody
      • K1C20_HUMAN antibody
      • K20 antibody
      • KA20 antibody
      • Keratin 20 antibody
      • keratin 20, type I antibody
      • keratin 21, rat, homolog of antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 20 antibody
      • Keratin-20 antibody
      • Keratin20 antibody
      • KRT 20 antibody
      • KRT 21 antibody
      • KRT20 antibody
      • KRT21 antibody
      • MGC35423 antibody
      • OTTHUMP00000164518 antibody
      • Protein IT antibody
      • type I cytoskeletal 20 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

      ab854 staining cytokeratin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

      ICC/IF image of ab854 stained Jeg3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab854, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)

      Immunofluorescent analysis of human colon epithelium, staining Cytokeratin 20 with ab854.

    • Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Flow Cytometry - Anti-Cytokeratin 20 antibody [Ks20.8] (ab854)
      Overlay histogram showing LoVo cells stained with ab854 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab854, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    ab854 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab854 は 3 報の論文で使用されています。

    • Wang Q  et al. Ketogenesis contributes to intestinal cell differentiation. Cell Death Differ 24:458-468 (2017). PubMed: 27935584
    • Fridriksdottir AJ  et al. Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture. Nat Commun 6:8786 (2015). PubMed: 26564780
    • Fang DD  et al. Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery. Br J Cancer : (2010). Flow Cyt ; Human . PubMed: 20332776

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin (Merkel cell carcinoma))
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    Yes - 0,2% Triton/PBS
    Specification
    skin (Merkel cell carcinoma)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2018

    Western blot abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin biopsie)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16%)
    Loading amount
    30 µg
    Specification
    skin biopsie
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 06 2018

    Western blot abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Colon Carcinoma)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    30 µg
    Specification
    Colon Carcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 22 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 20 antibody [Ks20.8]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Organoid)
    Permeabilization
    Yes - 0.3% Triton
    Specification
    Organoid
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 10 2016

    Question

    Dear colleagues

    One our customer bought Abcam cat# AB854 lot# GR94926-1, and she did note that this antibody was resospended in a almost red buffer: is it normal? Can you guarantee that this strange colour will not affect the performances?

    Please note that the same customer bough, several months ago, the same antibody (but with different lot number) and the buffer within was transparent.

    Thanks a lot

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that ab854 Anti-Cytokeratin 20 antibody [Ks20.8]is provided as tissue culture supernatant, which we would expect to have a pink-red coloration. Therefore, I would not expect this to affect the antibody.

    The last vial purchased may have been purified and provided in clear buffer.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    I am looking to purchase a monoclonal antibody to cytokeratin 20 for use in ELISA. Is this product, ab854, suitable for use in that application?

    Read More
    Answer

    This antibody has been tested for use in IHC, but to our knowledge has not yet been used in ELISA.

    Read More

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