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RabMAb

Anti-Cytokeratin 20 抗体 [EPR1622Y] (ab76126)

製品の概要

  • 製品名
    Anti-Cytokeratin 20 antibody [EPR1622Y]
    Cytokeratin 20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1622Y] to Cytokeratin 20
  • 由来種
    Rabbit
  • 特異性
    The immunogen of this antibody is 73% homolog with Mouse-Cytokeratin 20. This antibody gives positive results for mouse samples in Western Blot only. Therefore we do not recommend this antibody for mouse samples and do not cover mouse with our Abpromise guarantee.
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Rat, Goat, Human, Pig, Common marmoset
  • 免疫原

    Synthetic peptide within Human Cytokeratin 20 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Human small intestine lysate; human colon adenocarcinoma tissue.
  • 特記事項

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
WB 1/10000 - 1/50000. Predicted molecular weight: 48 kDa.
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
  • 組織特異性
    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
  • 翻訳後修飾
    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD20 antibody
    • CK 20 antibody
    • CK-20 antibody
    • CK20 antibody
    • Cytokeratin-20 antibody
    • Cytokeratin20 antibody
    • K1C20_HUMAN antibody
    • K20 antibody
    • KA20 antibody
    • Keratin 20 antibody
    • keratin 20, type I antibody
    • keratin 21, rat, homolog of antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 20 antibody
    • Keratin-20 antibody
    • Keratin20 antibody
    • KRT 20 antibody
    • KRT 21 antibody
    • KRT20 antibody
    • KRT21 antibody
    • MGC35423 antibody
    • OTTHUMP00000164518 antibody
    • Protein IT antibody
    • type I cytoskeletal 20 antibody
    see all

画像

  • Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Cytokeratin 20 with purified ab76126 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Anti-Cytokeratin 20 antibody [EPR1622Y] (ab76126) at 1/50000 dilution + human small intestine lysate at 10 µg

    Secondary
    goat anti-rabbit-HRP at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 44 kDa (why is the actual band size different from the predicted?)



    Primary: ab76126, 1/50000 dilution
    Sample: human small intestine lysate, 10 ug
    Secondary: goat anti-rabbit-HRP, 1/1000 dilution

  • ab76126 at 1/100 dilution staining Cytokeratin 20 in human colon adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
  • Overlay histogram showing LoVo cells stained with ab76126 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76126, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Primary: ab76126, 1/50000 dilution
    Sample: ab73640, 0.01 ug
    Secondary: ab97080, 1/5000 dilution

  • ab76126 showing positive staining in Colonic adenocarcinoma tissue.

  • ab76126 showing positive staining in Urinary bladder transitional carcinoma tissue.

  • ab76126 showing negative staining in Hepatocellular carcinoma tissue.

  • ab76126 showing negative staining in Breast carcinoma tissue.

  • Fluorescent immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using ab76126. Green-CK20 red-PI

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

参考文献

This product has been referenced in:
  • Yoshida T  et al. Three-dimensional organoid culture reveals involvement of Wnt/ß-catenin pathway in proliferation of bladder cancer cells. Oncotarget 9:11060-11070 (2018). Read more (PubMed: 29541396) »
  • Shangguan W  et al. Endothelium originated from colorectal cancer stem cells constitute cancer blood vessels. Cancer Sci 108:1357-1367 (2017). IHC-Fr . Read more (PubMed: 28421697) »

See all 18 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Urothelial cell)
Permeabilization
Yes - .1% Triton
Specification
Urothelial cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 25 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Urothelial cell)
Permeabilization
Yes - 0.1% Triton
Specification
Urothelial cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 25 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bladder cancer)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Bladder cancer
Blocking step
Casein (DAKO ­ Protein Block Serum-Free) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER Tris/HCL pH 9.0
Sample
Pig Tissue sections (Porcine Instestine)
Specification
Porcine Instestine
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

投稿 Aug 08 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Colon and bladder)
Specification
Colon and bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 27 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Small intestine ( Jejunum))
Specification
Small intestine ( Jejunum)
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Marmoset (common) Tissue sections (Jejunum)
Specification
Jejunum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Thank you for your enquiry regarding ab76126 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise a...

Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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