Alexa Fluor® 488 Anti-Cytokeratin 19 抗体 [EP1580Y] (ab192643)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP1580Y] to Cytokeratin 19
- Suitable for: Flow Cyt (Intra), ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]
Cytokeratin 19 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rabbit monoclonal [EP1580Y] to Cytokeratin 19 -
由来種
Rabbit -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: HeLa. ICC/IF KO: MCF7 cells (MCF7-KRT19 KO used as a negative cell line). Flow Cyt (intra): HeLa cells.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
解離定数(KD 値)
KD = 3.70 x 10 -10 M Learn more about KD -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1580Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (ab192980)
- HRP Anti-Cytokeratin 19 antibody [EP1580Y] (ab193600)
- Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free (ab195872)
- Alexa Fluor® 594 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203443)
- Alexa Fluor® 555 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203444)
- Alexa Fluor® 568 Anti-Cytokeratin 19 antibody [EP1580Y] (ab203445)
- APC Anti-Cytokeratin 19 antibody [EP1580Y] (ab224980)
- PE Anti-Cytokeratin 19 antibody [EP1580Y] (ab224981)
- Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (ab52625)
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192643の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/500.
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|
ICC/IF |
1/100.
Signal can be observed in cells fixed with either methanol or paraformaldehyde. |
特記事項 |
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Flow Cyt (Intra)
1/500. |
ICC/IF
1/100. Signal can be observed in cells fixed with either methanol or paraformaldehyde. |
ターゲット情報
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機能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
組織特異性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Present in hair follicles at all stages of development. -
ドメイン
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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参照データベース
- Entrez Gene: 3880 Human
- Entrez Gene: 16669 Mouse
- Omim: 148020 Human
- SwissProt: P08727 Human
- SwissProt: P19001 Mouse
- Unigene: 654568 Human
- Unigene: 439699 Mouse
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別名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
画像
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Immunofluorescence staining of Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel) using ab192643. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192643 at 1/500 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Flow Cytometry analysis of Cytokeratin-19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab192643 (red line).
The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192643, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Immunofluorescence staining of Cytokeratin 19 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using ab192643. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192643 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150120) at 2 μg/ml (shown in pseudo-color red).
Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab192643 は論文での使用が確認できていません。