Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Breast carcinoma)
Specification
Breast carcinoma
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10%
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS with 10% goat serum
Secondary antibody
Name
Non-Abcam antibody was used: Ultravision ONE HRP Polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This antibody is very well suited for staining of breast carcinomas (as well as the normal breast gland) in cryostat sections. We routinely use methanol fixation, but the antibody also works well with formaldehyde fixation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Rene Villadsen
Verified customer
投稿 Sep 25 2009