Biotin Anti-Cytokeratin 19 抗体 [A53-B/A2] (ab239265)
Key features and details
- Biotin Mouse monoclonal [A53-B/A2] to Cytokeratin 19
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Biotin Anti-Cytokeratin 19 antibody [A53-B/A2]
Cytokeratin 19 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [A53-B/A2] to Cytokeratin 19 -
由来種
Mouse -
標識
Biotin -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 19. (MCF7 human breast adenocarcinoma cell line).
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ポジティブ・コントロール
- Flow Cyt (Intra): MCF7 cells. WB: HT29 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. -
ポリ/モノ
モノクローナル -
クローン名
A53-B/A2 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab239265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use a concentration of 1 - 5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 44 kDa).
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特記事項 |
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Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 44 kDa). |
ターゲット情報
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機能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
組織特異性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Present in hair follicles at all stages of development. -
ドメイン
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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参照データベース
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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別名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
画像
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All lanes : Biotin Anti-Cytokeratin 19 antibody [A53-B/A2] (ab239265) at 2 µg/ml
Lane 1 : HT-29 cell lysate (reducing)
Lane 2 : MOLT-4 cell lysate (reducing)
Lane 3 : MOLT-4 cell lysate (non-reducing)
Lane 4 : HT-29 cell lysate (non-reducing)
Secondary
All lanes : IRDye800-conjugated streptavidin
Predicted band size: 44 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted? -
Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cell line labeling Cytokeratin 19 with ab239265 at 1/500 dilution (red), compared with a negative control. Intracellular staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab239265 は論文での使用が確認できていません。