Anti-Cytokeratin 18 抗体 [EPR17347] (ab181597)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17347] to Cytokeratin 18
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Cytokeratin 18 antibody [EPR17347]
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17347] to Cytokeratin 18 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Mouse colon, rat lung, mouse kidney, human fetal skin and mouse spleen tissue lysates, HeLa and F9 cell lysates. IHC-P: Human liver and colonic adenocarcinoma, mouse liver and rat kidney tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17347 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000. Detects a band of approximately 47, 45 kDa (predicted molecular weight: 47 kDa).
|
|
IHC-P | (3) |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/2000. Detects a band of approximately 47, 45 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
-
参照データベース
- Entrez Gene: 3875 Human
- Entrez Gene: 16668 Mouse
- Entrez Gene: 294853 Rat
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: P05784 Mouse
- SwissProt: Q5BJY9 Rat
- Unigene: 406013 Human
see all -
別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
-
Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on tumor cells of Human colon cancer. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on hepatocytes of Human liver. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on hepatocytes of mouse liver. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Cytokeratin 18 with ab181597 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. IHC showed membrane and cytoplasm staining on tubules of rat kidney. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
All lanes : Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/10000 dilution
Lane 1 : Mouse colon lysate
Lane 2 : Rat lung lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
-
All lanes : Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
-
Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/10000 dilution + Human fetal skin lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
-
Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/2000 dilution + F9 (Mouse embyro testicular cancer cell line) whole cell lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
The observed MW is consistent with what has been described in the literature (PMID:9298992).
-
All lanes : Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa whole cell lysate treated with 1uM staurosporine for 4 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 22,45,48 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds5% NFDM/TBST: Blocking and diluting buffer.
The 22 kDa cytokeratin 18 fragment was generated (lane 2) in the staurosporine-treated lysate that contained the active caspase 3.
-
All lanes : Anti-Cytokeratin 18 antibody [EPR17347] (ab181597) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa whole cell lysate treated with 50uM Z-VAD-FMK for 4 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds5% NFDM/TBST: Blocking and diluting buffer.
The generation of the 22kDa fragment was inhibited when the caspase 3 activity was blocked by the caspase inhibitor, Z-VAD-FMK.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (17)
ab181597 は 17 報の論文で使用されています。
- Del-Pozo J et al. The EDA-deficient mouse has Zymbal's gland hypoplasia and acute otitis externa. Dis Model Mech 15:N/A (2022). PubMed: 35107126
- Liu Q et al. ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway. Cell Death Discov 8:51 (2022). PubMed: 35136027
- Wang DH et al. Isolation and characterization mesenchymal stem cells from red panda (Ailurus fulgens styani) endometrium. Conserv Physiol 10:coac004 (2022). PubMed: 35211318
- Gao D et al. Transcriptional Feedback Loops in the Caprine Circadian Clock System. Front Vet Sci 9:814562 (2022). PubMed: 35478603
- Ali G & Abdelalim EM Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells. STAR Protoc 3:101613 (2022). PubMed: 35990735