Anti-Cytokeratin 18 抗体 [EPR1626] (ab133263)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1626] to Cytokeratin 18
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Cytokeratin 18 antibody [EPR1626]
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1626] to Cytokeratin 18 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1626 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133263の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/20.
|
|
WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
For unpurified use at 1/10,000 - 1/50,000 |
|
IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. 1:500 in human tissues / 1:1000 in rat tissues |
ICC/IF | (1) |
1/50.
For unpurified use at 1/250 - 1/500 |
特記事項 |
---|
Flow Cyt (Intra)
1/20. |
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). For unpurified use at 1/10,000 - 1/50,000 |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. 1:500 in human tissues / 1:1000 in rat tissues |
ICC/IF
1/50. For unpurified use at 1/250 - 1/500 |
ターゲット情報
-
機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
-
参照データベース
- Entrez Gene: 3875 Human
- Entrez Gene: 294853 Rat
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: Q5BJY9 Rat
- Unigene: 406013 Human
- Unigene: 103924 Rat
-
別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
-
Flow cytometry overlay histogram showing left MCF7 positive cells and right negative A375 stained with ab133263 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab133263) (1x 106 in 100μl at 0.04μg/ml (1/51000)) for 30min at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in MCF7 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labeling Cytokeratin 18 with Purified ab133263 at 1:500 dilution (0.23 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 18 with Purified ab133263 at 1/20 dilution (10µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
All lanes : Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/20000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 18 with Purified ab133263 at 1:50 dilution (2.26 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Alexa Fluor® 488 (ab206091) and Alexa Fluor® 647 (ab206269) conjugated versions are available for this clone.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Cytokeratin 18 with Purified ab133263 at 1:1000 dilution (0.113 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/1000 dilution (Purified) + Rat liver lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells labelling Cytokeratin 18 with ab133263 at 1/250.
-
Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue labelling Cytokeratin 18 with ab133263 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-Cytokeratin 18 antibody [EPR1626] (ab133263) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 48 kDa -
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Cytokeratin 18 with unpurified ab133263 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 80% methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
Immunohistochemical analysis of paraffin embedded Human breast tissue labelling Cytokeratin 18 with ab133263 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133263 showing positive staining in Prostatic carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133263 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133263 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133263 showing positive staining in Breast carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133263 showing positive staining in Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab133263 showing negative staining in Cervical squamous carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (25)
ab133263 は 25 報の論文で使用されています。
- Gao Z et al. LncRNA LINC02253 activates KRT18/MAPK/ERK pathway by mediating N6-methyladenosine modification of KRT18 mRNA in gastric cancer. Carcinogenesis 43:419-429 (2022). PubMed: 35136989
- Gonzalez ME et al. EZH2 T367 phosphorylation activates p38 signaling through lysine methylation to promote breast cancer progression. iScience 25:104827 (2022). PubMed: 35992062
- Palafox M et al. High p16 expression and heterozygous RB1 loss are biomarkers for CDK4/6 inhibitor resistance in ER+ breast cancer. Nat Commun 13:5258 (2022). PubMed: 36071033
- Mao Y et al. MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration. Reprod Sci N/A:N/A (2022). PubMed: 36303086
- Liu X et al. miR-155 promotes proliferation and epithelial-mesenchymal transition of MCF-7 cells. Exp Ther Med 21:218 (2021). PubMed: 33500705