Anti-Cytokeratin 18 抗体 [DA-7] (ab669)

Submit a review Q&A (2)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DA-7] (ab669)
  • Flow Cytometry - Anti-Cytokeratin 18 antibody [DA-7] (ab669)

Key features and details

  • Mouse monoclonal [DA-7] to Cytokeratin 18
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytokeratin 18 antibody [DA-7]
    Cytokeratin 18 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [DA-7] to Cytokeratin 18

  • 由来種

    Mouse

  • 特異性

    Human Cytokeratin peptide 18 (45 kDa).

  • アプリケーション

    適用あり: Flow Cyt, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue/ cell preparation (Human). (Breast carcinoma cell line PMC-42).

  • ポジティブ･コントロール

    • ELISA, WB, IP: MCF-7 human breast adenocarcinoma cell line lysate IHC-P: epithelial tissue (skin)

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • 組織特異性

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • 関連疾患

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • 配列類似性

    Belongs to the intermediate filament family.

  • 翻訳後修飾

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • 細胞内局在

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DA-7] (ab669)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DA-7] (ab669)
      Ab669 staining Human normal skin. Staining is localized to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Flow Cytometry - Anti-Cytokeratin 18 antibody [DA-7] (ab669)
      Flow Cytometry - Anti-Cytokeratin 18 antibody [DA-7] (ab669)
      Overlay histogram showing MCF7 cells stained with ab669 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab669, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献 (5)

    ab669 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab669 は 5 報の論文で使用されています。

    • Smolders J  et al. Tissue-resident memory T cells populate the human brain. Nat Commun 9:4593 (2018). IHC-P ; Human . PubMed: 30389931
    • Taylor-Papadimitriou J  et al. Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci 94 ( Pt 3):403-13 (1989). PubMed: 2483723
    • Vojtesek B  et al. Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha) 35:373-82 (1989). PubMed: 2483834
    • Lauerová L  et al. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7:495-504 (1988). PubMed: 2461901
    • Kovari­k J  et al. Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl 3:50-5 (1988). PubMed: 2463228

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Do you know the epitopes for these antibodies (amino acids)?

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    Answer

    The epitope has not been determined.

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    Question

    Hi Jennifer, I went over the website and home page for the protocol for ELISA. I am attaching the protocol for you. My concern with this that I have to work with the dilution profile (step 7) to check the conc. of cytokeratin 18 in my sample. Do you have purified Cytokeratin 18 with which I acn make serial dilutions for the standard curve to find out unknown cytokeratin 18 in my samples? If yes please give me cat. #. I can order either ab669 or ab 7797 as both are mouse monoclonal antibodies against cytokeratin 18. As a secondary I am going to order ab6728 (Rabbit polyclonal to mouse and conjugated to HRP. Can I use TMB substrate from any other company or you suggest to use only #ab8587 for ELISA? Since you have this antibody for Cytokeratin18 for ELISA, someone must have done in house experiment in your company. I will graetly appreciate if you can give me the protocol. A reply will be graetly appreciated as I would like to order these antibodies by next week. Thanks a lot. Gurpreet

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    Answer

    I enquired with the supplier of ab669 and ab7797 and unfortunately they do not have a specific ELISA protocol for either of these two antibodies. All we can recommend is to consult Abcam's general ELISA protocol. If you have any more questions or concerns, please contact us again.

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