Key features and details
- Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Sheep, Goat, Horse, Hamster, Cow, Cat, Dog, Human, Pig, Common marmoset
- Isotype: IgG1
製品名Anti-Cytokeratin 18 antibody [C-04]
Cytokeratin 18 一次抗体 製品一覧
製品の詳細Mouse monoclonal [C-04] to Cytokeratin 18
アプリケーション適用あり: IHC-P, Flow Cytmore details
種交差性交差種: Mouse, Rat, Sheep, Goat, Horse, Hamster, Cow, Cat, Dog, Human, Pig, Common marmoset
Tissue, cells or virus corresponding to Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
- IHC-P: Human skin tissue. Flow Cyt: HCT 116 cells.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
精製度Protein A purified
特記事項（精製）Purified from culture supernatant. Purity >95% by SDS-PAGE.
Our Abpromise guarantee covers the use of ab668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use 1µg for 106 cells.
Also see PMID 18946470.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
機能Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
組織特異性Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
関連疾患Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
配列類似性Belongs to the intermediate filament family.
翻訳後修飾Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
細胞内局在Cytoplasm > perinuclear region.
- Information by UniProt
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ab668 staining Cytokeratin 18 in the human hepatocellular carcinoma cell line HepG2 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (7:3) and blocked with 1% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS ) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated rabbit anti-mouse IgG polyclonal was used as the secondary antibody at 1/100 dilution.
ab668 (2 µg/ml) staining cytokeratin 18 in human skin tissue using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of sweat coils.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were immersed in 3% H2O2/methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX.
Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Overlay histogram showing HCT 116 (Human colorectal carcinoma cell line) cells stained with ab668 (red line).
The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT 116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
ab668 staining Cytokeratin 18 in cat lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Coloured arrowheads indicate positivity.
Good immunolabeling of bronchial tree epithelia (green), alveolar lining epithelium (red ).
Goblet cells are negative (asterisk).
Heavily stained structures are submucosal mucous glands.
ab668 staining cytokeratin 18 in mouse epithelium tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Composite image of d14 embryo shows developing epithelia.
Upper image: positive simple epithelium of renal tubules
Lower image: negative stratified epithelium of epidermis
ab668 staining Cytokeratin 18 in horse primary bronchial epithelial cells by Immunocytochemistry/ Immunofluorescence.
The cells were fixed in acetone and then blocked using 3% BSA for 10 hours at 4°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 22°C. The secondary antibody used was a goat anti-mouse conjugated to FITC used at a 1/200 dilution.
ab668 は 96 報の論文で使用されています。
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