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Anti-Cytokeratin 10 抗体 [RKSE60] (ab9025)

Reviews (4)Q&A (11)References (17)
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)

Key features and details

  • Mouse monoclonal [RKSE60] to Cytokeratin 10
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Pig, Zebrafish
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytokeratin 10 antibody [RKSE60]
    Cytokeratin 10 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [RKSE60] to Cytokeratin 10

  • 由来種

    Mouse

  • 特異性

    This antibody reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas

  • アプリケーション

    適用あり: IHC-Fr, ICC/IFmore details

  • 種交差性

    交差種: Pig, Zebrafish

  • 免疫原

    Full length native protein (purified) corresponding to Human Cytokeratin 10. Cytokeratins from the human epidermis.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    RKSE60

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9025の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (2)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 組織特異性

    Seen in all suprabasal cell layers including stratum corneum.

  • 関連疾患

    Defects in KRT10 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT10 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.

  • 配列類似性

    Belongs to the intermediate filament family.
  • Information by UniProt

  • 別名

    • BCIE antibody
    • BIE antibody
    • CK 10 antibody
    • CK-10 antibody
    • Cytokeratin-10 antibody
    • EHK antibody
    • K10 antibody
    • K1C10_HUMAN antibody
    • Keratin 10 antibody
    • Keratin 10 type I antibody
    • Keratin antibody
    • Keratin type i cytoskeletal 10 antibody
    • Keratin type I cytoskeletal 59 kDa antibody
    • Keratin-10 antibody
    • Keratin10 antibody
    • KPP antibody
    • KRT10 antibody
    • type I cytoskeletal 10 antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
    Immunohistochemistry on frozen section of swine skin: positive staining of the keratinizing layer
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [RKSE60] (ab9025)
    Immunofluorescence staining of a 7 days old zebrafish embryo

参考文献 (17)

ab9025 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9025 は 17 報の論文で使用されています。

  • Li J  et al. SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation. Nat Commun 12:784 (2021). PubMed: 33542242
  • Li J  et al. ELL Facilitates RNA Polymerase II-Mediated Transcription of Human Epidermal Proliferation Genes. J Invest Dermatol 141:1352-1356.e3 (2021). PubMed: 33157094
  • Ling J  et al. RAS-mediated suppression of PAR3 and its effects on SCC initiation and tissue architecture occur independently of hyperplasia. J Cell Sci 133:N/A (2020). PubMed: 33172988
  • Li J  et al. TEAD1 and TEAD3 Play Redundant Roles in the Regulation of Human Epidermal Proliferation. J Invest Dermatol 140:2081-2084.e4 (2020). PubMed: 32142794
  • Li J  et al. HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs. Nat Commun 10:4198 (2019). PubMed: 31519929
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 15 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 10 antibody [RKSE60]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (skin)
Permeabilization
Yes - 0.4% triton x-100
Specification
skin
Blocking step
IHC Blocking Buffer High Protein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jul 06 2020

Western blot abreview for Anti-Cytokeratin 10 antibody [RKSE60]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
adipose-derived stem cell culture media
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 29 2016

Western blot abreview for Anti-Cytokeratin 10 antibody [RKSE60]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
burn wound with stem cell treatment
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 May 20 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 10 antibody [RKSE60]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Acetone
Permeabilization
No
Blocking step
Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 20°C
Read More

DR. Vaughan Feisst

Verified customer

投稿 Sep 17 2010

Question

Oui l’anticorps neomarkers est un monoclonal de souris. Dans la fiche technique il n’y a pas d’application pour la cytométrie en flux pour cet anticorps, donc je ne l’ai pas testé en FACS…

Je vais aussi réfléchir de mon côté. Affaire à suivre.

Bonne soirée

Read More
Answer

Vous avez testé l'anticorps anti-cytokeratin 10 et l'isotype en Western Blot. Avez-vous également utilisé l'anticorps secondaire seul?
Je suis étonné que la bande observée avec l'isotype se retrouve au même poids moléculaire que le primaire. Cette bande étant proche des 50 kDa, je me demande si ce n'est pas un marquage non-spécifique du secondaire. Un controle "no primary" permettrait de déterminer quel anticorps se fixe à ce niveau de la membrane.

Je vaiscontinuer de réfléchir à votre problème.

Bonne fin de journée et bon week-end.

Read More

Question

Bonjour,



Voici le document rempli avec des résultats de FACS, les photos IF et le WB.

L’anticorps abcam marche en IF et WB, par contre l’isotype reconnait une bande de la même taille que K10 en WB…

Je n’ai pas testé l’isotype en IF, mais je pourrais éventuellement tester les deux AC en WB sur HEK pour voir si j’ai le même résultat qu’en FACS.



Dans l’attente d’une réponse de votre part

Read More
Answer

Mercipour votre réponse ainsi que pour le questionnaire rempli et détaillé.

Il m'est toujours impossible de trouver une explication définitive à votre problème.
Est-ce que l'anticorps anti-K10 est également un monoclonal de souris IgG1 ?
Est-ce que ce même anticorps marque les controles négatifs (HEK) en cytométrie?
Quel controle isotype utilisez-vous avec cet anticorps?

J'espère pouvoir trouver rapidement une explication et une résolution à votre problème. N'hésitez pas à me communiquer votre opinion sur la question.

Read More

Question

Problèmes en FACS et IFavec ab9025 (primaire) et ab18448 (controle isotype).
Anticorps couplés avec kit invitrogen "zenon"

controles négatifs : HEK

marquage positif obtenus dans tous les cas de figures.

Read More
Answer

En plus de vos résultats obtenus en Western Blot, vous serait-il possible de remplir et nous retourner le questionnaire ci-joint?

Les informations sur le protocole utilisé nous sera utile pour tenter de comprendre le problème rencontré avec les 2 anticorps ab9025 et ab18448.

Merci.

Read More

Question

Problèmes en FACS et IFavec ab9025 (primaire) et ab18448 (controle isotype).
Anticorps couplés
controles négatifs : HEK

marquage positif obtenus dans tous les cas de figures.

Read More
Answer

Merci de nous avoir contactés.

Je vous envoie comme convenu un email auquel vous pourrez répondre et joindre vos résultats en Western Blot avec les anticorps ab9025 et ab18448.
Votre réponse me sera alors automatiquement attribuée.

Read More

Question

what is the light chain of this antibody?

Read More
Answer



Le laboratoire vient de nous confirmer que la chaine légère du clone [RKSE60] est de type kappa.

Read More

Question

At what dilution the antibody has been used?

Read More
Answer

Thank you for your phone call. I found just the information in our records and am happy to confirm that the laboratory has obtained good results with this antibody at 1:100 – 1:200 for flow cytometry. I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.

Read More

1-10 of 15 Abreviews or Q&A

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