Key features and details
- Mouse monoclonal [AE1] to Cytokeratin 1
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle
- Isotype: IgG1
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.1% Sodium azide
Concentration information loading...
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab9286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/150 - 1/200.|
機能May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
組織特異性The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
関連疾患Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
配列類似性Belongs to the intermediate filament family.
翻訳後修飾Undergoes deimination of some arginine residues (citrullination).
細胞内局在Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
- Information by UniProt
- 67 kDa cytokeratin antibody
- CK-1 antibody
- CK1 antibody
ab9286 staining Cytokeratin 1 in rat normal skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.3. Samples were then blocked with 5% BSA for 20 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 1 hour at 27°C. An HRP-conjugated goat polyclonal was used as secondary antibody undiluted.
ab9286 は 12 報の論文で使用されています。
- Choo MK et al. The protein kinase p38a destabilizes p63 to limit epidermal stem cell frequency and tumorigenic potential. Sci Signal 11:N/A (2018). PubMed: 30301786
- Yin C et al. TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis. Sci Rep 4:5831 (2014). PubMed: 25060222
- Caballero-Franco C et al. Tuning of protein kinase circuitry by p38a is vital for epithelial tissue homeostasis. J Biol Chem 288:23788-97 (2013). PubMed: 23836897
- Spagnolo DV et al. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am J Clin Pathol 84:697-704 (1985). PubMed: 2416215
- Sun TT et al. Monoclonal antibody studies of mammalian epithelial keratins: a review. Ann N Y Acad Sci 455:307-29 (1985). PubMed: 2417518