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    cytohesin-2-antibody-10a12-ab2728.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Anti-Cytohesin 2 抗体 [10A12] (ab2728)

  • Datasheet
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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

Key features and details

  • Mouse monoclonal [10A12] to Cytohesin 2
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Cytohesin 2 protein (ab134606)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Cytohesin 2 antibody [10A12]
    Cytohesin 2 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [10A12] to Cytohesin 2
  • 由来種

    Mouse
  • 特異性

    Detects recombinant human cytohesin-2 (ARNO).
  • アプリケーション

    適用あり: ICC/IF, IHC-Pmore details
    適用なし: WB
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (His-tag) corresponding to Cytohesin 2.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 特記事項(精製)

    PEG purified IgG1
  • ポリ/モノ

    モノクローナル
  • クローン名

    10A12
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Cytohesin 2 protein (ab134606)

アプリケーション

Our Abpromise guarantee covers the use of ab2728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/20.
IHC-P 1/25 - 1/200.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能

      Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane.
    • 組織特異性

      Ubiquitous.
    • 配列類似性

      Contains 1 PH domain.
      Contains 1 SEC7 domain.
    • ドメイン

      The PH domain is necessary and sufficient for plasma membrane relocalization.
    • 細胞内局在

      Cell membrane. Cytoplasm. Both isoform 1 and isoform 2 are recruited to the cell membrane through its association with ARL4A, ARL4C and ARL4D. Requires also interaction with phosphoinositides for targeting to plasma membrane.
    • Target information above from: UniProt accession Q99418 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 9266 Human
      • Entrez Gene: 19158 Mouse
      • Omim: 602488 Human
      • SwissProt: Q99418 Human
      • SwissProt: P63034 Mouse
      • Unigene: 144011 Human
      • Unigene: 272130 Mouse
      • 別名

        • ARF exchange factor antibody
        • ARF nucleotide binding site opener antibody
        • ARF nucleotide-binding site opener antibody
        • Arno antibody
        • ARNO protein antibody
        • CLM2 antibody
        • CTS18 antibody
        • CTS18.1 antibody
        • CYH2_HUMAN antibody
        • Cyth2 antibody
        • Cytohesin 2 antibody
        • Cytohesin-2 antibody
        • MGC137537 antibody
        • MGC80440 antibody
        • PH antibody
        • PH, SEC7 and coiled-coil domain-containing protein 2 antibody
        • Pleckstrin homology Sec7 and coiled coil domains 2 antibody
        • Pleckstrin homology Sec7 and coiled coil domains protein 2 antibody
        • Protein ARNO antibody
        • PSCD2 antibody
        • PSCD2L antibody
        • PSCD2L, formerly antibody
        • SEC7 and coiled-coil domain-containing protein 2 antibody
        • Sec7 antibody
        • SEC7 homolog B antibody
        • Sec7B antibody
        • SEC7L antibody
        • Sec7p L antibody
        • Sec7p-like antibody
        • Sec7pL antibody
        see all

      画像

      • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
        Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

        Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

      • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
        Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

        Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

      • Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)
        Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

        Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human colon carcinoma tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human stomach tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

      プロトコール

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
    • 参考文献 (2)

      ab2728 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2728 は 2 報の論文で使用されています。

      • Yang MQ  et al. Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury. Hepatology 67:1056-1070 (2018). PubMed: 29059701
      • Zhu W  et al. Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy. J Clin Invest 127:4569-4582 (2017). PubMed: 29058688

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      There are currently no Customer reviews or Questions for ab2728.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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