Anti-Cytochrome P450 3A4/CYP3A4 抗体 (ab3572)
Key features and details
- Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytochrome P450 3A4/CYP3A4 antibody
Cytochrome P450 3A4/CYP3A4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Other Immunogen Type (His-tag) corresponding to Human Cytochrome P450 3A4/CYP3A4. Purified, His-tagged, full length human P450 3A4 fusion protein.
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ポジティブ･コントロール
- Rat liver lysate.
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特記事項
This product was previously labelled as Cytochrome P450 3A4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). A 46kDa band is detected in rat liver extracts. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. -
組織特異性
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). -
配列類似性
Belongs to the cytochrome P450 family. -
翻訳後修飾
Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1576 Human
- Omim: 124010 Human
- SwissProt: P08684 Human
- Unigene: 728751 Human
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別名
- 1,8-cineole 2-exo-monooxygenase antibody
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
see all
画像
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver with Skimmed milk
Lane 2 : Rat liver with Skimmed milk
Lysates/proteins at 30 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 57 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)ab3572 (1µg/ml) staining Cytochrome P450 3A4/CYP3A4 in human Liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasm of the plates of hepatic cells .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)Western blot of Cytochrome P450 3A4/CYP3A4 on rat liver extract using ab3572.
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)ab3572 at a dilution of 1/2000 staining Cytochrome P450 3A4/CYP3A4 in rat liver extract by Western blot.
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/500 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa, 29 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
プロトコール
参考文献 (21)
ab3572 は 21 報の論文で使用されています。
- Reed L et al. Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine. Toxicol Appl Pharmacol 366:64-74 (2019). PubMed: 30685480
- Zhou XY et al. Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro. Front Pharmacol 10:591 (2019). PubMed: 31214030
- Katsuda T et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. Elife 8:N/A (2019). PubMed: 31393263
- Lin Y et al. Bmal1 regulates circadian expression of cytochrome P450 3a11 and drug metabolism in mice. Commun Biol 2:378 (2019). PubMed: 31633069
- He Z et al. Chaihu-Shugan-San Reinforces CYP3A4 Expression via Pregnane X Receptor in Depressive Treatment of Liver-Qi Stagnation Syndrome. Evid Based Complement Alternat Med 2019:9781675 (2019). PubMed: 31781287
