Anti-Cytochrome P450 3A4/CYP3A4 抗体 (ab135813)

Reviews (2)Q&A (2)References (7)

製品の概要

  • 製品名

    Anti-Cytochrome P450 3A4/CYP3A4 antibody
    Cytochrome P450 3A4/CYP3A4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human Cytochrome P450 3A4/CYP3A4 aa 234-264 (internal sequence) conjugated to keyhole limpet haemocyanin.

  • ポジティブ･コントロール

    • Human liver tissue; CEM cells; NCI-H460 cell line lysates.

  • 特記事項

     This product was previously labelled as Cytochrome P450 3A4

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab135813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 57 kDa.

ターゲット情報

  • 機能

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.

  • 組織特異性

    Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).

  • 配列類似性

    Belongs to the cytochrome P450 family.

  • 翻訳後修飾

    Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).

  • 細胞内局在

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 1,8-cineole 2-exo-monooxygenase antibody
      • Albendazole monooxygenase antibody
      • Albendazole sulfoxidase antibody
      • CP33 antibody
      • CP34 antibody
      • CP3A4_HUMAN antibody
      • CYP3 antibody
      • CYP3A antibody
      • CYP3A3 antibody
      • CYP3A4 antibody
      • CYPIIIA3 antibody
      • CYPIIIA4 antibody
      • Cytochrome P450 3A3 antibody
      • Cytochrome P450 3A4 antibody
      • Cytochrome P450 family 3 subfamily A polypeptide 4 antibody
      • Cytochrome P450 HLp antibody
      • Cytochrome P450 NF-25 antibody
      • Cytochrome P450 subfamily IIIA polypeptide 4 antibody
      • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 antibody
      • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 antibody
      • Cytochrome P450-PCN1 antibody
      • Glucocorticoid inducible P450 antibody
      • HLP antibody
      • MGC126680 antibody
      • NF 25 antibody
      • NF25 antibody
      • Nifedipine oxidase antibody
      • P450 III steroid inducible antibody
      • P450 PCN1 antibody
      • P450, family III antibody
      • P450C3 antibody
      • P450PCN1 antibody
      • Quinine 3 monooxygenase antibody
      • Quinine 3-monooxygenase antibody
      • Taurochenodeoxycholate 6 alpha hydroxylase antibody
      • Taurochenodeoxycholate 6-alpha-hydroxylase antibody
      see all

    画像

    • Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813)
      Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813)
      All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813) at 1/50 dilution

      Lane 1 : NCI-H460 cell line lysates
      Lane 2 : NCI-H460 cell line lysates with blocking peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813)

      Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human liver tissue labelling Cytochrome P450 3A4/CYP3A4 with ab135813 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

    • Flow Cytometry - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813)
      Flow Cytometry - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab135813)

      Flow Cytometric analysis of CEM cells labelling Cytochrome P450 3A4/CYP3A4 with ab135813 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    参考文献

    This product has been referenced in:

    • Danoy M  et al. Optimized protocol for the hepatic differentiation of induced pluripotent stem cells in a fluidic microenvironment. Biotechnol Bioeng 116:1762-1776 (2019). Read more (PubMed: 30883676) »
    • Chang YT  et al. Role of CYP3A4 in bone marrow microenvironment-mediated protection of FLT3/ITD AML from tyrosine kinase inhibitors. Blood Adv 3:908-916 (2019). Read more (PubMed: 30898762) »
    See all 7 Publications for this product

    ab135813 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Cytochrome P450 3A4/CYP3A4 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    40 µg
    Specification
    HEPG2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 19 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytochrome P450 3A4/CYP3A4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblast)
    Permeabilization
    Yes - 0.3% Triton X-100 in blocking buffer
    Specification
    fibroblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 29 2018

    Question

    Thank you for your offer.

    I’m interested to test these 2 antibodies in ICC.

    Could you send me the discount code to order the antibodies?

    Read More
    Answer




    I am very pleased to hear you would like to accept our offer and testboth ab55840and ab135813in ICC. These codes will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    I would like to order some antibodies for ICC.

    I would like to know if the antibody ab135813 (CYP3A4) can be used for ICC and if the antibody ab55840 (CYP3A7) can be used for ICC?

    Thank you for your quick answer. If yes we will order both product on Monday.

    Read More
    Answer

    Thank you very much for your interest in our products.

    To our knowledge, bothab135813 and ab55840have not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buyab135813 and ab55840 now, test it in ICCand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per tested antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab135813 and ab55840 in ICC. I will then send a discount code. This code must be issued before purchasing ab135813 and ab55840 so please wait for my reply before ordering.

    2. Purchase ab135813 and ab55840 either by phone, fax, or online (www.abcam.com).

    3. Test it in ICC.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimery antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab135813 and ab55840 turn out to be unsuitable for ICC, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

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