Anti-Cytochrome P450 2B1 + 2B2 抗体 [b/e3 (9.14)] (ab22719)
![Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)](https://www.abcam.co.jp/ps/products/22/ab22719/Images/ab22719-196681-anti-cytochrome-p450-2b1-2b2-antibody-b-e3-914-immunoprecipitation.jpg "Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)")
Key features and details
- Mouse monoclonal [b/e3 (9.14)] to Cytochrome P450 2B1 + 2B2
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
-
製品名
Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)]
Cytochrome P450 2B1 + 2B2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [b/e3 (9.14)] to Cytochrome P450 2B1 + 2B2 -
由来種
Mouse -
特異性
This product does not react with Rat CYP 1A1, 1A2, 2A1, 2C6, 2C7, 2C11, 4A2 and 4A3 -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Mouse -
免疫原
The details of the immunogen for this antibody are not available.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS
Buffer: PBS -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
b/e3 (9.14) -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22719の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
|
| 特記事項 |
|---|
|
IP
Use a concentration of 5 µg/ml. |
ターゲット情報
-
関連性
The Cytochrome P450 superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. -
細胞内局在
Membrane bound, endoplasmic reticulum -
別名
- Cyp2b 1 antibody
- Cyp2b 2 antibody
- Cyp2b1 antibody
see all
画像
-
Immunoprecipitation - Anti-Cytochrome P450 2B1 + 2B2 antibody [b/e3 (9.14)] (ab22719)
Cytochrome P450 2B1 + 2B2 was immunoprecipitated using 0.5mg Mouse Liver tissue, 5µg of Mouse monoclonal to Cytochrome P450 2B1 + 2B2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22719.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 56kDa; Cytochrome P450 2B1 + 2B2
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab22719 は 2 報の論文で使用されています。
- Chanyshev MD et al. Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. Toxicol Mech Methods N/A:1-8 (2020). PubMed: 32508177
- Chou LY et al. Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38. Int J Mol Sci 21:N/A (2020). PubMed: 33003599